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Full-Text Articles in Physics
Contributions Of Vibrational Spectroscopy To Virology: A Review, Iqra Chaudhary, Naomi Jackson, Denise Denning, Luke O'Neill, Hugh Byrne
Contributions Of Vibrational Spectroscopy To Virology: A Review, Iqra Chaudhary, Naomi Jackson, Denise Denning, Luke O'Neill, Hugh Byrne
Articles
Vibrational spectroscopic techniques, both infrared absorption and Raman scattering, are high precision, label free analytical techniques which have found applications in fields as diverse as analytical chemistry, pharmacology, forensics and archeometrics and, in recent times, have attracted increasing attention for biomedical applications. As analytical techniques, they have been applied to the characterisation of viruses as early as the 1970s, and, in the context of the coronavirus disease 2019 (COVID-19) pandemic, have been explored in response to the World Health Organisation as novel methodologies to aid in the global efforts to implement and improve rapid screening of viral infection. This review …
In Vitro Analysis Of Immersed Human Tissues By Raman Microspectroscopy, Franck Bonnier, A. Mehmood, Peter Knief, Aidan Meade, Helen Lambkin, Kathleen Flynn, V. Mcdonagh, C. Healy, T. C. Lee, Fiona Lyng, Hugh Byrne
In Vitro Analysis Of Immersed Human Tissues By Raman Microspectroscopy, Franck Bonnier, A. Mehmood, Peter Knief, Aidan Meade, Helen Lambkin, Kathleen Flynn, V. Mcdonagh, C. Healy, T. C. Lee, Fiona Lyng, Hugh Byrne
Articles
Raman microspectroscopy is a powerful tool for the analysis of tissue sections, providing a molecular map of the investigated samples. Nevertheless, data pre-processing and, particularly, the removal of the broad background to the spectra remain problematic. Indeed, the physical origin of the background has not been satisfactorily determined. Using 785 nm as source in a confocal geometry, it is demonstrated for the example of the protein kappa-elastin that the background and resulting quality of the recorded spectrum are dependent on the morphology of the sample. Whereas a fine powder yields a dominant broad background, compressed pellets and solution-cast thin films …