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Preparation Of Oriented, Fully Hydrated Lipid Samples For Structure Determination Using X-Ray Scattering., Stephanie Tristram-Nagle
Preparation Of Oriented, Fully Hydrated Lipid Samples For Structure Determination Using X-Ray Scattering., Stephanie Tristram-Nagle
Prof. Stephanie Tristram-Nagle Ph.D.
This chapter describes a method of sample preparation called "the rock and roll method," which is basically a solvent evaporation technique with controlled manual sample movement during evaporation of solvent from lipid/solvent mixtures that produces well-oriented thick stacks of about 2000 lipid bilayers. Many lipid types have been oriented using different solvent mixtures that balance solubilization of the lipid with uniform deposition of the lipid solution onto solid substrates. These well-oriented thick stacks are then ideal samples for collection of both X-ray diffraction data in the gel phase and X-ray diffuse scattering data in the fluid phase of lipids. The …
Lipid Bilayer Structure., John Nagle, Stephanie Tristram-Nagle
Lipid Bilayer Structure., John Nagle, Stephanie Tristram-Nagle
Prof. Stephanie Tristram-Nagle Ph.D.
Fluctuations, inherent in flexible and biologically relevant lipid bilayers, make quantitative structure determination challenging. Shortcomings in older methods of structure determination have been realized and new methodologies have been introduced that take fluctuations into account. The large uncertainty in literature values of lipid bilayer structural parameters is being reduced.