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Full-Text Articles in Physics
Sub-Micron Parallel Laser Direct-Write, Christina Othon, Arnoldo Laracuente, H Ladoucuer, Bradley Ringeisen
Sub-Micron Parallel Laser Direct-Write, Christina Othon, Arnoldo Laracuente, H Ladoucuer, Bradley Ringeisen
Christina M Othon
We have developed a modified laser induced forward transfer (LIFT) technique which allows for the parallel deposition of hundreds of sub-micron features. The approach utilizes a self-assembled monolayer of monodisperse polystyrene microspheres as the focusing element. A monolayer of close-packed microspheres is formed on top of an ultra-thin quartz support (25 m thick), and a metallic thin film is then deposited on the underside of the quartz. This approach is different from previous parallel microsphere-assisted LIFT experiments that required the deposition of metal directly onto the surface of the periodic microsphere structure. For this study, an 800 nm, 130 fs …
Single-Cell Printing To Form Three-Dimensional Lines Of Olfactory Ensheathing Cells, Christina Othon, Xingjia Wu, Juanita Anders, Bradley Ringeisen
Single-Cell Printing To Form Three-Dimensional Lines Of Olfactory Ensheathing Cells, Christina Othon, Xingjia Wu, Juanita Anders, Bradley Ringeisen
Christina M Othon
Biological laser printing (BioLP™) is a unique tool capable of printing high resolution two- and three-dimensional patterns of living mammalian cells, with greater than 95% viability. These results have been extended to primary cultured olfactory ensheathing cells (OECs), harvested from adult Sprague-Dawley rats. OECs have been found to provide stimulating environments for neurite outgrowth in spinal cord injury models. BioLP is unique in that small load volumes (~µLs) are required to achieve printing, enabling low numbers of OECs to be harvested, concentrated and printed. BioLP was used to form several 8 mm lines of OECs throughout a multilayer hydrogel scaffold. …