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Development Of A Two-Photon Laser Scanning Microscope, Jess Hollenbaugh
Development Of A Two-Photon Laser Scanning Microscope, Jess Hollenbaugh
Student Research Symposium
The objective of this project was to convert a Sarastro 2000 confocal laser scanning microscope into a system capable of imaging using two-photon excitation (TPE) fluorescence for the use of the PSU biology department. TPE microscopy operates on the ability of fluorophores to accept two photons each with half the energy of a desired transition in a single quantum event via a virtual energy state and then emit a higher energy photon upon relaxation. This is preferable to single-photon excitation (SPE) due to the lower energy photons causing less damage to delicate biological samples. The adaptation process included physically altering …