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Contribution Of Membrane Elastic Energy To Rhodopsin Function, Olivier Soubias, Walter E. Teague, Kirk G. Hines, Drake C. Mitchell, Klaus Gawrisch
Contribution Of Membrane Elastic Energy To Rhodopsin Function, Olivier Soubias, Walter E. Teague, Kirk G. Hines, Drake C. Mitchell, Klaus Gawrisch
Physics Faculty Publications and Presentations
We considered the issue of whether shifts in the metarhodopsin I (MI)-metarhodopsin II (MII) equilibrium from lipid composition are fully explicable by differences in bilayer curvature elastic stress. A series of six lipids with known spontaneous radii of monolayer curvature and bending elastic moduli were added at increasing concentrations to the matrix lipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and the MI-MII equilibrium measured by flash photolysis followed by recording UV-vis spectra. The average area-per-lipid molecule and the membrane hydrophobic thickness were derived from measurements of the 2H NMR order parameter profile of the palmitic acid chain in POPC. For the series of ethanolamines …