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Full-Text Articles in Physics
Microfluidic Separation Of Live And Dead Yeast Cells Using Reservoir-Based Dielectrophoresis, Saurin Patel, Daniel Showers, Pallavi Vedantam, Tzuen-Rong Tzeng, Shizhi Qian, Xiangchun Xuan
Microfluidic Separation Of Live And Dead Yeast Cells Using Reservoir-Based Dielectrophoresis, Saurin Patel, Daniel Showers, Pallavi Vedantam, Tzuen-Rong Tzeng, Shizhi Qian, Xiangchun Xuan
Mechanical & Aerospace Engineering Faculty Publications
Separating live and dead cells is critical to the diagnosis of early stage diseases and to the efficacy test of drug screening, etc. This work demonstrates a novel microfluidic approach to dielectrophoretic separation of yeast cells by viability. It exploits the cell dielectrophoresis that is induced by the inherent electric field gradient at the reservoir-microchannel junction to selectively trap dead yeast cells and continuously separate them from live ones right inside the reservoir. This approach is therefore termed reservoir-based dielectrophoresis (rDEP). It has unique advantages as compared to existing dielectrophoretic approaches such as the occupation of zero channel space and …
Negative Dielectrophoretic Capture Of Bacterial Spores In Food Matrices, Mehti Koklu, Seungkyung Park, Suresh D. Pillai, Ali Beskok
Negative Dielectrophoretic Capture Of Bacterial Spores In Food Matrices, Mehti Koklu, Seungkyung Park, Suresh D. Pillai, Ali Beskok
Mechanical & Aerospace Engineering Faculty Publications
A microfluidic device with planar square electrodes is developed for capturing particles from high conductivity media using negative dielectrophoresis (n-DEP). Specifically, Bacillus subtilis and Clostridium sporogenes spores, and polystyrene particles are tested in NaCl solution (0.05 and 0.225 S/m), apple juice (0.225 S/m), and milk (0.525 S/m). Depending on the conductivity of the medium, the Joule heating produces electrothermal flow (ETF), which continuously circulates and transports the particles to the DEP capture sites. Combination of the ETF and n-DEP results in different particle capture efficiencies as a function of the conductivity. Utilizing 20 μm height DEP chambers, “almost complete” and …
Studies Of Breakdown In A Pressurized Rf Cavity, M. Bastaninejad, A. A. Elmustafa, C. M. Ankenbrandt, A. Moretti, M. Popovic, K. Yonehara, D. M. Kaplan, M. Alsharo'a, P. M. Hanlet, R. P. Johnson, M. Kuchnir, D. Newsham, D. V. Rose, C. Thoma, D. R. Welch
Studies Of Breakdown In A Pressurized Rf Cavity, M. Bastaninejad, A. A. Elmustafa, C. M. Ankenbrandt, A. Moretti, M. Popovic, K. Yonehara, D. M. Kaplan, M. Alsharo'a, P. M. Hanlet, R. P. Johnson, M. Kuchnir, D. Newsham, D. V. Rose, C. Thoma, D. R. Welch
Mechanical & Aerospace Engineering Faculty Publications
Microscopic images of the surfaces of metallic electrodes used in high-pressure gas-filled 805 MHz RF cavity experiments [1] have been used to investigate the mechanism of RF breakdown [2]. The images show evidence for melting and boiling in small regions of ∼10 micron diameter on tungsten, molybdenum, and beryllium electrode surfaces. In these experiments, the dense hydrogen gas in the cavity prevents electrons or ions from being accelerated to high enough energy to participate in the breakdown process so that the only important variables are the fields and the metallic surfaces. The distributions of breakdown remnants on the electrode surfaces …