Physics Commons^™

Bayesian Analysis Of Single Molecule Fluorescence Microscopy Data, Mohamadreza Fazel

Physics & Astronomy ETDs

The diffraction limit can be circumvent by creating and exploiting independent behaviors of the sample at lengths scale below the diffraction limit. In fluorescence microscopy, the independence arises from individual fluorescent labels switching between dark and fluorescence states. The fluorophores can then be localized employing the generated sparse image frames. Finally, the resulting list of coordinates is utilized to generate high resolution images or to gain quantitative insight into the underlying biological structures. Therefore image processing and post-processing are essential stages of SMLM techniques.

In this dissertation, Reversible Jump Markov Chain Monte Carlo was employed to implement Bayesian analysis of …

High-Throughput Automated Multi-Target Super-Resolution Imaging, Farzin Farzam

Physics & Astronomy ETDs

Super-resolution microscopy techniques developed through the past few decades enable us to surpass the classical diffraction limit of light, and thus open new doors to investigate the formerly inaccessible world of nanometer-sized objects. Most importantly, by using super-resolution microscopy, one can visualize sub-cellular structures in the range of 10 to 200 nm. At this range, we can investigate exciting problems in biology and medicine by visualizing protein-protein interactions and spatiotemporal analysis of structures of interest on the surface or inside cells. These techniques (collectively known as nanoscopy) have a high impact on understanding and solving biological questions. This dissertation starts …