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Binding Of ΒL-Crystallin With Models Of Animal And Human Eye Lens-Lipid Membrane, Preston Hazen, Geraline Trossi-Torres, Nawal K. Khadka, Raju Timsina, Laxman Mainali
Binding Of ΒL-Crystallin With Models Of Animal And Human Eye Lens-Lipid Membrane, Preston Hazen, Geraline Trossi-Torres, Nawal K. Khadka, Raju Timsina, Laxman Mainali
Physics Faculty Publications and Presentations
Several discoveries show that with age and cataract formation, β-crystallin binds with the lens membrane or associates with other lens proteins, which bind with the fiber cell plasma membrane, accompanied by light scattering and cataract formation. However, how lipids (phospholipids and sphingolipids) and cholesterol (Chol) influence β-crystallin binding to the membrane is unclear. This research aims to elucidate the role of lipids and Chol in the binding of β-crystallin to the membrane and the membrane’s physical properties (mobility, order, and hydrophobicity) with β-crystallin binding. We used electron paramagnetic resonance (EPR) spin-labeling methods to investigate the binding of βL-crystallin …