Physics Commons^™

Temperature Dependent Protein-Chromophore Hydrogen Bond Dynamics In The Far-Red Fluorescent Proteins By Using Molecular Dynamics Simulation And Quantum Calculationtemperature Dependent Protein-Chromophore Hydrogen Bond Dynamics In The Far-Red Fluorescent Proteins By Using Molecular Dynamics Simulation And Quantum Calculation, Chandra Prasad Dhakal

FIU Electronic Theses and Dissertations

Fluorescent proteins are valuable tools as biochemical markers in molecular and cell biology research for studying cellular processes. Red Fluorescent Proteins (RFPs) are highly desirable for in vivo applications in living cell imaging because they absorb and emit light in the red region of the spectrum where cellular autofluorescence. Naturally occurring fluorescent proteins with emission peaks in this region of the spectrum occur in dimeric or tetrameric forms. For their use as biochemical markers, several monomeric variants of RFP have been developed which include mCherry, dsRed, and mStrawberry. Far red-emitting FPs with large Stokes shift are especially valuables for in …