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Full-Text Articles in Physics
Involvement Of The Extrinsic And Intrinsic Pathways In Ultraviolet B-Induced Apoptosis Of Corneal Epithelial Cells, John L. Ubels, Courtney D. Glupker, Mark P. Schotanus, Loren D. Haarsma
Involvement Of The Extrinsic And Intrinsic Pathways In Ultraviolet B-Induced Apoptosis Of Corneal Epithelial Cells, John L. Ubels, Courtney D. Glupker, Mark P. Schotanus, Loren D. Haarsma
University Faculty Publications and Creative Works
The goal of this study was to elucidate the pathway by which UVB initiates efflux of K+ and subsequently apoptosis in human corneal limbal epithelial (HCLE) cells. The initial focus of the study was on the extrinsic pathway involving Fas. HCLE cells transfected with Fas siRNA were exposed to 80-150 mJ/cm2 UVB and incubated in culture medium with 5.5 mM K+. Knockdown of Fas resulted in limited reduction in UVB-induced caspase-8 and -3 activity. Patch-clamp recordings showed no difference in UVB-induced normalized K+ currents between Fas transfected and control cells. Knockdown of caspase-8 had no effect on the activation of …
Potassium Ion Fluxes In Corneal Epithelial Cells Exposed To Uvb, John L. Ubels, Rachel E. Van Dyken, Julienne R. Louters, Mark P. Schotanus
Potassium Ion Fluxes In Corneal Epithelial Cells Exposed To Uvb, John L. Ubels, Rachel E. Van Dyken, Julienne R. Louters, Mark P. Schotanus
University Faculty Publications and Creative Works
The goal of this study was to investigate the efflux of K+ from human corneal limbal epithelial cells (HCLE) exposed to ambient levels of UVB, which is known to cause apoptosis, and to examine the effect of K+ channel blockers on loss of potassium induced by UVB. HCLE cells were exposed to 100-200 mJ/cm2 UVB, followed by incubation in culture media with 5.5-100 mM K+, BDS-1, Ba2+ or ouabain. To measure intracellular cations, cells were washed in 280 mM sucrose and lysed in DI water. K+ and Na+ levels in lysates were measured by ion chromatography. HCLE cells showed maximal …
Potassium Ion Fluxes In Corneal Epithelial Cells Exposed To Uvb, John L. Ubels, Rachel E. Van Dyken, Julienne R. Louters, Mark P. Schotanus
Potassium Ion Fluxes In Corneal Epithelial Cells Exposed To Uvb, John L. Ubels, Rachel E. Van Dyken, Julienne R. Louters, Mark P. Schotanus
University Faculty Publications and Creative Works
The goal of this study was to investigate the efflux of K+ from human corneal limbal epithelial cells (HCLE) exposed to ambient levels of UVB, which is known to cause apoptosis, and to examine the effect of K+ channel blockers on loss of potassium induced by UVB. HCLE cells were exposed to 100-200 mJ/cm2 UVB, followed by incubation in culture media with 5.5-100 mM K+, BDS-1, Ba2+ or ouabain. To measure intracellular cations, cells were washed in 280 mM sucrose and lysed in DI water. K+ and Na+ levels in lysates were measured by ion chromatography. HCLE cells showed maximal …