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Potassium Ion Fluxes In Corneal Epithelial Cells Exposed To Uvb, John L. Ubels, Rachel E. Van Dyken, Julienne R. Louters, Mark P. Schotanus
Potassium Ion Fluxes In Corneal Epithelial Cells Exposed To Uvb, John L. Ubels, Rachel E. Van Dyken, Julienne R. Louters, Mark P. Schotanus
University Faculty Publications and Creative Works
The goal of this study was to investigate the efflux of K+ from human corneal limbal epithelial cells (HCLE) exposed to ambient levels of UVB, which is known to cause apoptosis, and to examine the effect of K+ channel blockers on loss of potassium induced by UVB. HCLE cells were exposed to 100-200 mJ/cm2 UVB, followed by incubation in culture media with 5.5-100 mM K+, BDS-1, Ba2+ or ouabain. To measure intracellular cations, cells were washed in 280 mM sucrose and lysed in DI water. K+ and Na+ levels in lysates were measured by ion chromatography. HCLE cells showed maximal …
Potassium Ion Fluxes In Corneal Epithelial Cells Exposed To Uvb, John L. Ubels, Rachel E. Van Dyken, Julienne R. Louters, Mark P. Schotanus
Potassium Ion Fluxes In Corneal Epithelial Cells Exposed To Uvb, John L. Ubels, Rachel E. Van Dyken, Julienne R. Louters, Mark P. Schotanus
University Faculty Publications and Creative Works
The goal of this study was to investigate the efflux of K+ from human corneal limbal epithelial cells (HCLE) exposed to ambient levels of UVB, which is known to cause apoptosis, and to examine the effect of K+ channel blockers on loss of potassium induced by UVB. HCLE cells were exposed to 100-200 mJ/cm2 UVB, followed by incubation in culture media with 5.5-100 mM K+, BDS-1, Ba2+ or ouabain. To measure intracellular cations, cells were washed in 280 mM sucrose and lysed in DI water. K+ and Na+ levels in lysates were measured by ion chromatography. HCLE cells showed maximal …