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Full-Text Articles in Physics
Development Of 3d In Vitro Tissue Models For The Analysis Of Solar Radiation Damage Of Skin, Ulises Lopez Gonzalez
Development Of 3d In Vitro Tissue Models For The Analysis Of Solar Radiation Damage Of Skin, Ulises Lopez Gonzalez
Doctoral
The aim of this work was to investigate changes to the molecular composition and conformation of HaCaT cells as a result of simulated solar radiation in a 3D in vitro skin model by Raman spectroscopy. The process to achieve this goal was performed in three main stages: (1) optimisation of the working concentration and volume of two 3D membranes, used as a structural support in the skin model; (2) the construction of the 3D in vitro skin model and; (3) the investigation of the dose-dependent effects of solar radiation on HaCaT cells in the skin model in comparison with the …
Multivariate Statistical Methodologies Used In In-Vitro Raman Spectroscopy: Simulations And Applications For Drug And Nanoparticle Interactions, Mark Edward Keating
Multivariate Statistical Methodologies Used In In-Vitro Raman Spectroscopy: Simulations And Applications For Drug And Nanoparticle Interactions, Mark Edward Keating
Doctoral
Raman spectroscopy is a growing technology in the fields of in-vitro drug and nanoparticle screening. The label free capability provided by vibrational spectroscopy, as well as the ability of the technique to probe the chemical nature of samples, makes it a good candidate for use in these fields. Crucial to the progress of these methods is the development and validation of robust and accurate multivariate statistical analysis protocols. In this thesis, both established and novel methods are examined using both real and simulated datasets. In particular, simulated datasets are used to validate and assess the accuracy of these methods in …
Advancing Vibrational Spectroscopy For Cellular And Sub Cellular Analysis: Raman Spectroscopy As An In Vitro Chemotherapeutic Screeening And Assessment Protocol, Zeineb Farhane
Doctoral
Vibrational spectroscopy, including Raman micro-spectroscopy, has attracted considerable attention over the last few years, as a powerful, non-invasive tool for clinical applications, especially in cancer diagnosis, in vivo and ex vivo. As a molecular fingerprinting technique with optical resolution, Raman micro-spectroscopy is able to monitor biochemical processes, drug uptake, efficacy and mode of action and mechanisms of interaction of chemotherapeutic drugs at a subcellular level. In vitro applications may be more strategically achievable, and can help guide drug design and discovery, and eventually evaluate To this end, different lung cell lines were used and Raman micro-spectroscopy was coupled to valuable …
Refraction In Mozambique: Evaluations Of Practice And Development Of Competency Frameworks For Eye Care Personnel, Kajal Shah
Doctoral
Purpose The development of competency- based education for optometrists and mid-level eye-care personnel has been identified as an important component in the elimination of avoidable blindness and vision impairment. The Mozambique Eye care Project (MEP) is a multi-institutional collaboration, which seeks to facilitate greater access to training in eye health professions, which will ultimately contribute to providing affordable and accessible eye care within the public health system in Mozambique. An important tenet of the MEP is to develop and enhance the refraction training of all existing (ophthalmic technicians) and new eye care personnel (four-year training of optometrists and eighteen-month training …
The Role Of Colour Duplex Ultrasound In The Surveillance Of Patients Undergoing Endovascular Aneurysm Repair, Cleona Gray
The Role Of Colour Duplex Ultrasound In The Surveillance Of Patients Undergoing Endovascular Aneurysm Repair, Cleona Gray
Doctoral
No abstract provided.
Potential Of Vibrational Spectroscopy In The Diagnosis Of Human Tumours., Eoghan O'Faolain
Potential Of Vibrational Spectroscopy In The Diagnosis Of Human Tumours., Eoghan O'Faolain
Doctoral
Just fewer than 20,000 people are annually diagnosed with some form of cancer in Ireland and one in three people are likely to contract some form of cancer by age 74. With the number of cases increasing at an annual rate of 2%, the early detection and treatment of cancer is becoming increasingly important. Both IR and Raman spectroscopy offer the potential for real time, quantitative detection of cancer and even precancer. This study investigates the potential of Raman and Fourier transform infrared, both benchtop and synchrotron spectroscopies for the detection of cervical cancer. The tissue was classified and its …