Physics Commons^™

Sensitive Fluorescence Detection Using A Camera From The Gaming Industry, Brian L. Van Hoozen Jr.

Lawrence University Honors Projects

The detection limit for fluorescence imaging has been improved by more than two orders of magnitude by a modulation technique that minimizes the influence of noise. Since most noise sources have a 1/f dependence, higher modulation frequencies result in less noise in the detected signal. Typical cameras used for imaging have frame rates of 50 or 100 hertz which is too low for substantial noise reduction. However, new time-of-flight cameras developed for the gaming industry have modulation frequencies of up to 20 MHz, allowing for a substantial reduction in noise in the detected signal. In this study, the improved detection …