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Full-Text Articles in Physics
Oligomerization Of The Sterile-2 G-Protein Coupled Receptor In Yeast Cells In The Presence And Absence Of Alpha-Factor Pheromone Using Fluorescence Spectroscopy And Forster Resonance Energy Transfer Analysis, Joel David Paprocki
Theses and Dissertations
G-protein-coupled receptors (GPCRs) are the largest family of receptors that respond to a wide variety of extracellular stimuli, including molecular ligands such as odorants, neurotransmitters, and hormones, as well as physical agents sigh as light and pressure. The stimulation event results in initiating conformational changes in the structure of the receptor, which further results in the release of the heterotrimeric G-protein; the latter has a variety of functions within signaling pathways in cellular biology. The GPCR explored in this investigation is the Sterile 2 α-factor receptor (Ste2), whose natural function is that of a yeast mating pheromone receptor. Its natural …
Two-Photon Microscopy For Biomedical Studies, Yassel Acosta
Two-Photon Microscopy For Biomedical Studies, Yassel Acosta
Open Access Theses & Dissertations
Fluorescence optical microscopy is one of the most important tools to investigate biological samples and to observe structures in living cells. Based on the principle of the simultaneous absorption of two-photons and subsequent emission of fluorescent light we have developed a Two-Photon Laser Scanning Fluorescence Microscope that allow us to perform optical sectioning and three-dimensional reconstructions of structures in biological samples such as dopaminergic clusters of neurons and tracheal branches in Drosophila brain. The spatial confinement of the excitation light to the focal plane combined with the temporal compression of photons into short packets during femtosecond pulses make our system …