Physics Commons^™

Positron Emission Tomography In Oncology And Environmental Science, Samantha Delaney

Dissertations, Theses, and Capstone Projects

The last half century has played witness to the onset of molecular imaging for the clinical assessment of physiological targets. While several medical imaging modalities allow for the visualization of the functional and anatomical properties of humans and living systems, few offer accurate quantitation and the ability to detect biochemical processes with low-administered drug mass doses. This limits how physicians and scientists may diagnose and treat medical issues, such as cancer, disease, and foreign agents.

A promising alternative to extant invasive procedures and suboptimal imaging modalities to assess the nature of a biological environment is the use of positron emission …

Simulation Of Polymerization On Surfaces: Implications For Abiogenesis, Sylvia Greene

Macalester Journal of Physics and Astronomy

How did life arise from the prebiotic conditions of the early earth? This problem has vexed scientists for decades with no consensus on its solution. Significant spontaneous formation of biopolymers such as proteins and nucleic acids in the aqueous phase appears to be improbable due to thermodynamic constraints. It has been proposed that mineral surfaces could have served as a catalyst for the initial formation of biopolymers. However, the feasibility of this mechanism has not been thoroughly studied. In this study, a particle simulation of polypeptide formation on surfaces is developed to assess the feasibility of this mechanism. Elementary processes …

Redesign Of X-Ray Irradiator To Expand Research Of Low-Dose X-Ray Radiation, Lauren Ulbrich

All Theses

The effects of high-dose radiation are well-documented, and are understood to be harmful to the human body. On the other hand, the effects of low-dose radiation are much less understood. There is debate over whether or not there are negative, positive, or no effects at all from low-dose radiation. One of the reasons why these effects are not well known is because there aren't as many studies done on this type of radiation. It is a lot harder to classify the effects of radiation at low doses due to many external factors. However, the Clemson Medical Physics lab has been …

Computational Modeling Of Retinal Damage Thresholds, Payton Hoffman, Eos Shapland, Alan Enriquez

SACAD: John Heinrichs Scholarly and Creative Activity Days

The Scalable Effects Simulation Environment(SESE), is the computational Biophysics software contracted by Nanohmics we used for our research. The goal was to find the minimum energy necessary to damage the retina from 2 laser sources, a 532 nm He-Ne source, and Supercontinuum Laser(SCL) source at 400-1400nm. We needed a 10 times damage ratio for significance. Our data did not show this trend.

Float Like A Butterfly, Sting Like A Bee!, Kobe D. Rome

SACAD: John Heinrichs Scholarly and Creative Activity Days

By injecting an electron into the empty pie* molecular orbital (LUMO) of Amino Acids in gas phase, we measure the Vertical Attachment Energies (VAEs) for the formation of short-lived anion states of these species using electron transmission spectroscopy (ETS). Our ETS study, a first of its kind to measure the VAE for the simplest of proteins, Glycine-Glycine (Gly-Gly), is currently in progress. Based on our previous measurements for several Amino Acids including Glycine, we expect a common range of attachment energy (1.50 – 2.00 eV) for Gly-Gly.

Predicting Biomolecular Properties And Interactions Using Numerical, Statistical And Machine Learning Methods, Elyssa Sliheet

Mathematics Theses and Dissertations

We investigate machine learning and electrostatic methods to predict biophysical properties of proteins, such as solvation energy and protein ligand binding affinity, for the purpose of drug discovery/development. We focus on the Poisson-Boltzmann model and various high performance computing considerations such as parallelization schemes.

Spectralomics – Towards A Holistic Adaptation Of Label Free Spectroscopy, Hugh Byrne

Articles

Vibrational spectroscopy, largely based on infrared absorption and Raman scattering techniques, is much vaunted as a label free approach, delivering a high content, holistic characterisation of a sample, with demonstrable applications in a broad range of fields, from process analytical technologies and preclinical drug screening, to disease diagnostics, therapeutics, prognostics and personalised medicine. However, in the analysis of such complex systems, a trend has emerged in which spectral analysis is reduced to the identification of individual peaks, based on reference tables of assignments derived from literature, which are then interpreted as biomarkers. More sophisticated analysis attempts to unmix the spectrum …

6d Single-Fluorogen Orientation-Localization Microscopy For Elucidating The Architecture Of Beta-Sheet Assemblies And Biomolecular Condensates, Tingting Wu, Weiyan Zhou, Jai S. Rudra, Rohit V. Pappu, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

We develop six-dimensional single-molecule orientation-localization microscopy (SMOLM) to measure the 3D positions and 3D orientations simultaneously of single fluorophores. We show how careful optimization of phase and polarization modulation components can encode phase, polarization, and angular spectrum information from each fluorescence photon into a microscope’s dipole-spread function. We used the transient binding and blinking of Nile red (NR) to characterize the helical structure of fibrils formed by designed amphipathic peptides, KFE8^L and KFE8^D, and the pathological amyloid-beta peptide Aβ42. We also deployed merocyanine 540 to uncover the interfacial architectures of biomolecular condensates.

A Causal Inference Approach For Spike Train Interactions, Zach Saccomano

Dissertations, Theses, and Capstone Projects

Since the 1960s, neuroscientists have worked on the problem of estimating synaptic properties, such as connectivity and strength, from simultaneously recorded spike trains. Recent years have seen renewed interest in the problem coinciding with rapid advances in experimental technologies, including an approximate exponential increase in the number of neurons that can be recorded in parallel and perturbation techniques such as optogenetics that can be used to calibrate and validate causal hypotheses about functional connectivity. This thesis presents a mathematical examination of synaptic inference from two perspectives: (1) using in vivo data and biophysical models, we ask in what cases the …

Synthesis Of Cu-Cy Nanoparticles For Treating Pfas In Water, Ligang Wang

Physics Theses

This work aims to address the cumulative harm caused by perfluoroalkyl and polyfluoroalkyl substances (PFAS) in water to human health and the ecological environment around the world. Reducing or removing PFAS pollution is an urgent need. Cu-Cy nanoparticles are photosensitizers, which were developed for photodynamic therapy of cancer in the first place. In this work, we use Cu-Cy for the catalytic degradation of PFAS. Synthesis of Cu-Cy nanoparticles in deionized water at 90 degrees Celsius is a low-cost and efficient method. We demonstrated the detailed process of synthesizing Cu-Cy nanoparticles. Under ultraviolet illumination, the synthesized Cu-Cy nanoparticles turned yellow as …