Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Physics
Detection And Diagnosis Of Bacterial Pathogens In Blood And Urine Using Laser-Induced Breakdown Spectroscopy, Emma J.M. Blanchette
Detection And Diagnosis Of Bacterial Pathogens In Blood And Urine Using Laser-Induced Breakdown Spectroscopy, Emma J.M. Blanchette
Electronic Theses and Dissertations
The aim of this thesis is to expand on and improve the existing techniques used for detecting and identifying bacterial pathogens in clinical specimens with laser-induced breakdown spectroscopy (LIBS). Specifically, the existing experimental procedures, including bacterial sample preparation and data acquisition, as well as the data analysis with chemometric algorithms were investigated. Substantial reductions in LIBS background signal were achieved by implementing rigorous cleaning steps and the introduction of the use of ultrapure water. Following this, a database of LIBS spectra was acquired from specimens of E. coli, S. aureus, E. cloacae, M. smegmatis, and P. …