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2,3,7,8-Tetrachlorodibenzo-P-Dioxin Induces Apoptotic Cell Death And Cytochrome P4501a Expression In Developing Fundulus Heteroclitus Embryos, Barbara Holland Toomey, Susan Bello, Mark E. Hahn, Susannah Cantrell, Peggy Wright, Donald E. Tillitt, Richard T. Di Giulio

United States Geological Survey: Staff Publications

Fundulus heteroclitus embryos were exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) during early development using nanoinjection or water bath exposure. TCDD caused developmental abnormalities that included hemorrhaging, loss of vascular integrity, edema, stunted development and death. The LC₅₀ and LD₅₀ of TCDD for Fundulus embryos were ~19.7 ± 9.5 pg TCDD/µl (water bath) and 0.25 ± 0.09 ng TCDD/g embryo (nanoinjection). To identify a possible cause for these developmental abnormalities we analyzed the effects of TCDD on apoptotic cell death and cytochrome P4501A (CYP1A) expression in the embryos. TCDD exposure increased apoptotic cell death in several tissues including brain, …