Environmental Health and Protection Commons^™

Microbial Community Function And Bacterial Pathogen Composition In Pit Latrines In Peri-Urban Malaw, Savanna K. Smith, Benjamin B. Risk, Rochelle H. Holm, Elizabeth Tilley, Petros Chigwechokha, Drew Capone, Joe Brown, Francis L. De Los Reyes Iii

Faculty Scholarship

Despite the widespread global reliance on pit latrines as improved sanitation systems, the decomposition of waste within pit latrines is poorly understood. One area needing elucidation is the characterization and function of microbial communities within pit latrines. To address this gap, we characterized the microbial communities of 55 lined pit latrines at three sampling layers from two communities in peri-urban Malawi. The microbial communities of the fecal sludge samples were analyzed for beta diversity, pathogen presence, and functional profiling. Household surveys were conducted and used to compare microbial community patterns to household characteristics and pit latrine use patterns. Compared to …