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Detection And Characterization Of Vibrio Vulnificus And Vibrio Parahaemolyticus Isolates: Pentaplex Pcr Assay And Its Application., Nabanita Bhattacharyya
Detection And Characterization Of Vibrio Vulnificus And Vibrio Parahaemolyticus Isolates: Pentaplex Pcr Assay And Its Application., Nabanita Bhattacharyya
LSU Master's Theses
The aim of the study was to develop an effective multiplex PCR protocol for simultaneous, rapid detection and characterization of two potential pathogens, Vibrio vulnificus and Vibrio parahaemolyticus and to enumerate their abundance in the environment. A Pentaplex PCR (pPCR) assay condition was developed with a combination of two species- and three pathogenic- specific PCR primer sets to simultaneously detect and characterize bacterial isolates for virulent/ non- virulent strains of V. parahaemolyticus and V. vulnificus. The pPCR assay was validated by three methods. First pPCR was tested on 300 bacterial isolates comprising of 7 reference strains, 117 V. vulnificus, 30 …