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United States Department of Commerce: Staff Publications

2012

Mass spectrometry

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Full-Text Articles in Environmental Sciences

Metabolite Profiles In Starved Diporeia Spp. Using Liquid Chromatography-Mass Spectrometry (Lc-Ms) Based Metabolomics, Suman Maity, Amber Jannasch, Jiri Adamec, Michael Gribskov, Thomas F. Nalepa, Tomas O. Höök, Maria S. Sepúlveda Jan 2012

Metabolite Profiles In Starved Diporeia Spp. Using Liquid Chromatography-Mass Spectrometry (Lc-Ms) Based Metabolomics, Suman Maity, Amber Jannasch, Jiri Adamec, Michael Gribskov, Thomas F. Nalepa, Tomas O. Höök, Maria S. Sepúlveda

United States Department of Commerce: Staff Publications

The holarctic amphipod Diporeia spp. was historically the most abundant benthic macroinvertebrate in the offshore region of the Laurentian Great Lakes basin. However, since the 1990’s, the numbers of Diporeia have declined precipitously throughout the region. Competition for food with introduced dreissenid mussels may be partly to blame for this decline. Thus, a better understanding of how Diporeia responds and adjust to starvation is needed. For this purpose, we used liquid chromatography (LC) coupled with time-of-flight mass spectrometry (TOFMS) to study the metabolite profiles of Diporeia during starvation. Diporeia were collected from Lake Michigan, brought to the laboratory and starved …


Starvation Causes Disturbance In Amino Acid And Fatty Acid Metabolism In Diporeia, Suman Maity, Amber Jannasch, Jiri Adamec, Thomas Nalepa, Tomas O. Höök, Maria S. Sepúlveda Jan 2012

Starvation Causes Disturbance In Amino Acid And Fatty Acid Metabolism In Diporeia, Suman Maity, Amber Jannasch, Jiri Adamec, Thomas Nalepa, Tomas O. Höök, Maria S. Sepúlveda

United States Department of Commerce: Staff Publications

The benthic amphipod Diporeia spp. was once the predominant macroinvertebrate in deep, offshore regions of the Laurentian Great Lakes. However, since the early 1990s, Diporeia populations have steadily declined across the area. It has been hypothesized that this decline is due to starvation from increasing competition for food with invasive dreissenid mussels. In order to gain a better understanding of the changes in Diporeia physiology during starvation, we applied two-dimensional gas chromatography coupled with time of flight mass spectrometry (GCXGC/TOF-MS) for investigating the responses in Diporeia metabolome during starvation. We starved Diporeia for 60 days and collected five organisms every …