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Full-Text Articles in Environmental Sciences
Trace Metal Availability Affects Greenhouse Gas Emissions And Microbial Functional Group Abundance In Freshwater Wetland Sediments, George Giannopoulos, Katherine R. Hartop, Bonnie L. Brown, Bongkeun Song, Lars Elsgaard, Rima B. Franklin
Trace Metal Availability Affects Greenhouse Gas Emissions And Microbial Functional Group Abundance In Freshwater Wetland Sediments, George Giannopoulos, Katherine R. Hartop, Bonnie L. Brown, Bongkeun Song, Lars Elsgaard, Rima B. Franklin
VIMS Articles
We investigated the effects of trace metal additions on microbial nitrogen (N) and carbon (C) cycling using freshwater wetland sediment microcosms amended with micromolar concentrations of copper (Cu), molybdenum (Mo), iron (Fe), and all combinations thereof. In addition to monitoring inorganic N transformations (NO3–, NO2–, N2O, NH4+) and carbon mineralization (CO2, CH4), we tracked changes in functional gene abundance associated with denitrification (nirS, nirK, nosZ), dissimilatory nitrate reduction to ammonium (DNRA; nrfA), and methanogenesis (mcrA). With regards to …
Composition And Bioavailability Of Effluent Dissolved Organic Nitrogen, Quinn Nicole Roberts
Composition And Bioavailability Of Effluent Dissolved Organic Nitrogen, Quinn Nicole Roberts
Dissertations, Theses, and Masters Projects
Cultural eutrophication, the overproduction of phytoplankton biomass in response to increased nutrient inputs directly associated with human activities, is a major threat to the health of Chesapeake Bay. Strict regulations, which require a reduction in nutrient loading from all sources, have been a key component to restoration efforts. Water reclamation facilities (WRFs), which discharge effluent containing nitrogen (N) and other nutrients into receiving waters, have implemented upgrades in an effort to comply with regulations. These improvements have decreased the concentration of highly labile dissolved inorganic N (DIN), leaving behind significant concentrations of dissolved organic N (DON) whose bioavailability, and therefore …
An Evaluation Of Norovirus Persistence In Estuarine Water And Of Methods For Its Detection In Treated Sewage Effluent, Margaret H. Fagan
An Evaluation Of Norovirus Persistence In Estuarine Water And Of Methods For Its Detection In Treated Sewage Effluent, Margaret H. Fagan
Dissertations, Theses, and Masters Projects
No abstract provided.
The Infection Dynamics Of Pav1 In The Caribbean Spiny Lobster Panulirus Argus, Caiwen Li
The Infection Dynamics Of Pav1 In The Caribbean Spiny Lobster Panulirus Argus, Caiwen Li
Dissertations, Theses, and Masters Projects
Panulirus argus Virus 1 (PaV1) is an emerging disease in Caribbean spiny lobster Panulirus argus. It is considered a threat to the lobster industry in the Florida Keys. In order to understand the infection dynamics of the PaV1 virus in the lobster host, a sensitive and specific fluorescence in situ hybridization (FISH) assay was developed for diagnosis of PaV1 in lobsters. The lower limit of detection using the 110-bp DNA probe in a dot-blot hybridization for PaV1 was 10 pg of cloned DNA template and 10 ng of genomic DNA extracted from hemolymph of diseased spiny lobster. The probe specifically …
Photochemical Production Of Dissolved Inorganic Nitrogen And Primary Amines From Dissolved Organic Nitrogen In Waters Of Two Estuaries And Adjacent Surficial Groundwaters, Dj Koopmans, Da Bronk
Photochemical Production Of Dissolved Inorganic Nitrogen And Primary Amines From Dissolved Organic Nitrogen In Waters Of Two Estuaries And Adjacent Surficial Groundwaters, Dj Koopmans, Da Bronk
VIMS Articles
Recent studies have shown that in a number of humic-rich surface waters in North America, NH4+ is released when dissolved organic matter (DOM) is exposed to sunlight. However, photochemical NH4+ production has not been observed in all surface waters, and factors that contribute to it are not well understood. We hypothesized that the presence or absence of NH4+ photoproduction may be affected by the light exposure history of DOM, The present study was undertaken to determine whether DOM from surficial groundwaters, with minimal light exposure history, would produce labile nitrogen (N) photoproducts more consistently, In this study, estuarine surface waters …
Changes In Bacterioplankton Metabolic Capabilities Along A Salinity Gradient In The York River Estuary, Virginia, Usa, G. E. Schultz, H. W. Ducklow
Changes In Bacterioplankton Metabolic Capabilities Along A Salinity Gradient In The York River Estuary, Virginia, Usa, G. E. Schultz, H. W. Ducklow
VIMS Articles
Changes in metabolic capabilities of bacterial communities along the estuarine salinity gradient may affect the extent of organic matter processing and bacterial growth and accumulation during transit through the system; As part of a larger study of estuarine microbial processes, we attempted to quantify differences in bacterial community structure using Biolog plates. Biolog GN plates (Biolog, Inc., Hayward, CA, USA) were used to determine differences in bacterioplankton community metabolic potential. Biolog GN microplates are 96-well microtiter plates in which each well contains an individual carbon source as well the redox dye tetrazolium violet. As bacteria grow and oxidize each substrate, …
Characterization Of Iron-Bearing Solids Used By Naturally-Occurring Microbes In The Anaerobic Degradation Of Hydrocarbons, David C. Powell
Characterization Of Iron-Bearing Solids Used By Naturally-Occurring Microbes In The Anaerobic Degradation Of Hydrocarbons, David C. Powell
Dissertations, Theses, and Masters Projects
No abstract provided.
Estimation Of Bacterial Respiration And Growth Efficiency In The Ross Sea, Antarctica, C. A. Carlson, N. R. Bates, H. W. Ducklow, D. A. Hansell
Estimation Of Bacterial Respiration And Growth Efficiency In The Ross Sea, Antarctica, C. A. Carlson, N. R. Bates, H. W. Ducklow, D. A. Hansell
VIMS Articles
Seawater cultures were conducted in large volume (36 l) gas impermeable tri-laminate bags for the purpose of empirically deriving bacterial growth efficiency (BGE) and carbon conversion factors (CCF) in the south central Ross Sea. This experimental design allowed for concomitant measurements of metabolic reactants (loss of total and dissolved organic carbon [TOC and DOG]) and products (gain of total carbon dioxide [TCO2] and bacterial biomass) to be made from a single incubation vessel. Some previous studies have relied on proxy measurements (e.g. O-2, H-3-thymidine incorporation and cell abundance) to determine BGE and CCF rather than direct carbon measurements. Our experimental …
Bacterial Growth In Experimental Plankton Assemblages And Seawater Cultures From The Phaeocystis Antarctica Bloom In The Ross Sea, Antarctica, H. W. Ducklow, C. Carlson, Walker O. Smith Jr.
Bacterial Growth In Experimental Plankton Assemblages And Seawater Cultures From The Phaeocystis Antarctica Bloom In The Ross Sea, Antarctica, H. W. Ducklow, C. Carlson, Walker O. Smith Jr.
VIMS Articles
A series of seawater culture experiments was carried out during the Phaeocystis antarctica bloom in the Ross Sea polynya (76.5 degrees S, 180 degrees W; November to December 1994 and December 1995 to January 1996) to examine bacterioplankton growth and derive empirical factors for estimating bacterial production rates. Bacterial growth was exponential over 3 to 10 d in all experiments, at rates of ca 0.1 to 0.7 d(-1), even in persistently cold waters (-2 to + 1 degrees C). Growth rates were lower in the early part of the bloom (early to mid-November) and highest during the period of peak …
Frna Coliphages As A Viral Indicator Of Sewage Pollution In An Estuary, David Miller Boyd
Frna Coliphages As A Viral Indicator Of Sewage Pollution In An Estuary, David Miller Boyd
Dissertations, Theses, and Masters Projects
No abstract provided.
Effects Of Selected Nitrogen-Containing Aromatic Compounds (Ncacs) On Physiological Properties In Escherichia Coli, W. James Catallo Iii
Effects Of Selected Nitrogen-Containing Aromatic Compounds (Ncacs) On Physiological Properties In Escherichia Coli, W. James Catallo Iii
Dissertations, Theses, and Masters Projects
This research examined the effects of quinoline and 4-azafluorene on respiratory electron transport rate (ET), outer membrane permeability and topology, oxygen consumption, and viable cell density in Escherichia coli cell suspensions. ET was estimated spectrophotometrically using INT (2-(p-iodophenyl)-3-(p-nitrophenyl)-5-(phenyl)-2H-tetrazolium chloride, which is reduced in vivo to a red colored formazan (INTF). Both test compounds caused anomalous dose-response behavior in INT assays: in a defined window of doses, ET rates near or above the controls were observed. These doses showed altered INT reduction kinetics, decreased cellular oxygen demand, and decreased viable cell densities. Experiments with E. coli spheroplast preparations, gram(+) cells, and …
Chitinoclastic Bacteria From The Mid-Atlantic Shelf: Distribution, Taxonomy, And Effect Of Oil On Growth And Chitin Degradation, Darbie Anne Lister
Chitinoclastic Bacteria From The Mid-Atlantic Shelf: Distribution, Taxonomy, And Effect Of Oil On Growth And Chitin Degradation, Darbie Anne Lister
Dissertations, Theses, and Masters Projects
No abstract provided.
Bacterial Response To Crude Oil Spillage In A Salt Marsh, Russell Paul Herwig
Bacterial Response To Crude Oil Spillage In A Salt Marsh, Russell Paul Herwig
Dissertations, Theses, and Masters Projects
No abstract provided.