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Full-Text Articles in Environmental Sciences

Recruitment And Post-Settlement Mortality Of The Soft-Shell Clam, Mya Arenaria, Shantelle Landry Jan 2021

Recruitment And Post-Settlement Mortality Of The Soft-Shell Clam, Mya Arenaria, Shantelle Landry

Dissertations, Theses, and Masters Projects

The soft-shell clam, Mya arenaria, is a benthic, filter-feeding, infaunal clam typically found in intertidal and shallow subtidal waters. Chesapeake Bay stocks of M. arenaria have been depleted since the 1960s due to various factors including predation, temperature, low recruitment, habitat loss, disease mortalities, and commercial harvest. As an important prey item for many commercial species, low abundances of these clams are mostly the result of the voracious appetite of the blue crab, Callinectes sapidus. In addition to predation, summer water temperatures in the Chesapeake Bay are likely driving the low abundances of M. arenaria, as water temperatures commonly surpass …


Composition And Bioavailability Of Effluent Dissolved Organic Nitrogen, Quinn Nicole Roberts Jan 2020

Composition And Bioavailability Of Effluent Dissolved Organic Nitrogen, Quinn Nicole Roberts

Dissertations, Theses, and Masters Projects

Cultural eutrophication, the overproduction of phytoplankton biomass in response to increased nutrient inputs directly associated with human activities, is a major threat to the health of Chesapeake Bay. Strict regulations, which require a reduction in nutrient loading from all sources, have been a key component to restoration efforts. Water reclamation facilities (WRFs), which discharge effluent containing nitrogen (N) and other nutrients into receiving waters, have implemented upgrades in an effort to comply with regulations. These improvements have decreased the concentration of highly labile dissolved inorganic N (DIN), leaving behind significant concentrations of dissolved organic N (DON) whose bioavailability, and therefore …


Partitioning Of Hydrophobic Organic Contaminants And Microbial Communities On Microplastics, Kelley Ann Uhlig Jan 2019

Partitioning Of Hydrophobic Organic Contaminants And Microbial Communities On Microplastics, Kelley Ann Uhlig

Dissertations, Theses, and Masters Projects

Microplastic contamination of aquatic environments has only recently caught the attention of scientists, regulators and the public. Microplastics are typically more recalcitrant than naturally occurring polymers and so have the potential to cause a range of issues, including increased exposure of marine life to chemical contaminants sorbed to or leached from microplastics, negative impacts due to ingestion of microplastics by biota, and the potential to carry and transport pathogenic and invasive species long distances. Bio-based, bio-degradable polymers have begun to gain market share as an alternative to traditional petrochemical-based plastics, but not much is known about their impacts in marine …


Human Dimensions Of A Participatory, Collaborative Modeling Process - Oysterfutures, Taylor Dawn Goelz Jan 2019

Human Dimensions Of A Participatory, Collaborative Modeling Process - Oysterfutures, Taylor Dawn Goelz

Dissertations, Theses, and Masters Projects

Participatory, collaborative modeling processes represent a unique decision-making technique within natural resources management that allows for the combination of stakeholder involvement with the analytical and predictive power of scientific models. The continued use of participatory modeling within decision-making processes depends in part upon the willingness of stakeholders to participate. Continued participation of stakeholders is key to the persistence and overall success of these processes, and yet limited information exists concerning the impacts of these processes on participants. The consideration of human dimensions advances our understanding of the design and function of participatory modeling processes, including their ability to create consensus …


Ecosystem Services Of Restored Oyster Reefs In A Chesapeake Bay Tributary: Abundance And Foraging Of Estuarine Fishes, Bruce William Pfirrmann Jan 2017

Ecosystem Services Of Restored Oyster Reefs In A Chesapeake Bay Tributary: Abundance And Foraging Of Estuarine Fishes, Bruce William Pfirrmann

Dissertations, Theses, and Masters Projects

Oyster reef restoration may enhance the production of ecologically or economically important fish species, an ecosystem service, by providing refuge and foraging habitat. Predicting the effects of oyster habitat restoration on fisheries production in Chesapeake Bay requires a better understanding of fish habitat use, trophic dynamics, and the processes leading to production on a habitat-scale. The objective of this thesis was to evaluate the influence of restored subtidal oyster reefs on the abundance and foraging patterns of mobile estuarine fishes. Specifically, I compared the 1) abundance, 2) stomach fullness, 3) diet composition, and 4) daily consumption rate of fishes collected …


Influence Of Structural Complexity And Location On The Habitat Value Of Restored Oyster Reefs, Melissa Ann Karp Jan 2016

Influence Of Structural Complexity And Location On The Habitat Value Of Restored Oyster Reefs, Melissa Ann Karp

Dissertations, Theses, and Masters Projects

In the Chesapeake Bay, < 1% of the historic oyster population remains, and efforts have been increasing to restore oysters and the services they provide. Building reefs that successfully provide ecosystem services–especially habitat and foraging grounds–may require different restoration techniques than those previously used, and success may depend on reef morphology (complexity), location, and environmental conditions. Salinity and habitat complexity are two important factors that may interact to effect benthic communities and predator-prey interactions on restored reefs. The goals of this project were: (1) Characterize the benthic communities on restored oyster reefs in lower Chesapeake Bay, and (2) examine the effects of structural complexity and salinity on benthic communities and predator-prey interactions. A two-year field survey of restored reefs was carried out in four rivers in lower Chesapeake Bay to characterize faunal communities on restored reefs and to quantify the effect of reef complexity on faunal communities. A laboratory mesocosm experiment was conducted to examine the effect of reef complexity on predator foraging. In total, 61 macrofaunal species were identified among all samples, and restored reefs supported on average, 6,169 org/m2 and 67.88 g-AFDW/m2. There were significant differences in the community composition and diversity among the rivers, and salinity was the environmental factor that best explained the observed differences in species composition across the rivers. Salinity and rugosity (i.e., structural complexity) both positively affected diversity, while salinity negatively affected macrofaunal abundance and biomass. Oyster density and rugosity positively affected macrofaunal biomass, and oyster density positively affected mud crab, polychaete, and mussel densities. In the mesocosm experiment, predator foraging, measured by proportion and number of prey consumed, was significantly reduced in the presence of oyster shell structure. However, predators were able to consume more prey when prey density was increased, even in the presence of oyster shell structure. These results combine to enhance our understanding of the benefits of increased habitat complexity for both prey and predators on restored oyster reefs. Increasing complexity worked to increase the abundance, biomass, and diversity of organisms inhabiting restored reefs, and even though predator consumption was reduced in the presence of structure compared to non-structured habitat, predators were able to consume more prey individuals when prey density was increased. Therefore, increasing the structure of oyster reef habitat may benefit prey species by providing refuge habitat, and benefit predators by providing an increased abundance of available prey items.


Influence Of Perkinsus Marinus Infection And Oyster Health On Levels Of Human-Pathogenic Vibrios In Oysters, Lydia M. Bienlien Jan 2016

Influence Of Perkinsus Marinus Infection And Oyster Health On Levels Of Human-Pathogenic Vibrios In Oysters, Lydia M. Bienlien

Dissertations, Theses, and Masters Projects

The eastern oyster Crassostrea virginica is an ecologically and commercially important species whose natural populations have been devastated by overharvesting, habitat destruction, and disease, but the rapid growth of oyster aquaculture has shown potential to restore the economic significance of this species. A key threat to the growth and sustainability of oyster aquaculture is the association of human-pathogenic Vibrio bacteria with product marketed for raw consumption. Two Vibrio species, Vibrio vulnificus and Vibrio parahaemolyticus, are the causes of the highest rates of seafood consumption-related mortality and gastrointestinal illness, respectively. Identification of the factors influencing V. vulnificus and V. parahaemolyticus prevalence …


The Effects Of Drifting Fish Aggregating Devices On Bycatch In The Tropical Tuna Purse Seine Fisheries In The Atlantic And Indian Oceans, Julia Snouck-Hurgronje Jan 2016

The Effects Of Drifting Fish Aggregating Devices On Bycatch In The Tropical Tuna Purse Seine Fisheries In The Atlantic And Indian Oceans, Julia Snouck-Hurgronje

Dissertations, Theses, and Masters Projects

Thousands of floating objects, known as drifting fish aggregating devices (dFADs), are released every year by commercial tropical tuna purse seine vessels in the three equatorial oceans to aggregate tuna and increase catch. The escalation in the number of dFADs deployed over the last three decades has caused changes in fishing effort that are poorly reflected in traditional indices of purse seine effort and catch per unit of effort (CPUE). In addition, concerns have been raised regarding the impacts of such high numbers of dFADs being deployed on both catch and bycatch species. I studied two aspects of dFAD deployments …


Modeling The Formation Of Periodic Hypoxia In Partially Mixed Estuaries And Its Response To Oligotrophication And Climate Change, Samuel J. Lake Jan 2013

Modeling The Formation Of Periodic Hypoxia In Partially Mixed Estuaries And Its Response To Oligotrophication And Climate Change, Samuel J. Lake

Dissertations, Theses, and Masters Projects

The development of hypoxia represents one of the most common and ecologically detrimental effects of anthropogenic nutrient enrichment in coastal marine ecosystems. Due to the physiological importance of oxygen as a key component of metabolic processes, the development and persistence of hypoxia can reduce the distribution of important species, modify food webs, decrease diversity and richness, and sub-lethally affect growth and reproductive rates. While many recent studies have focused on the global increase in hypoxia and highlighted the need for nutrient reduction strategies, some key processes associated with hypoxia remain understudied. of particular importance is the resolution of the major …


Effects Of Anthropogenic Stressors And Changes In Biodiversity On Lower Chesapeake Bay, Va Usa Seagrass Systems, Rachael E. Blake Jan 2012

Effects Of Anthropogenic Stressors And Changes In Biodiversity On Lower Chesapeake Bay, Va Usa Seagrass Systems, Rachael E. Blake

Dissertations, Theses, and Masters Projects

Anthropogenic stressors are increasingly changing conditions in coastal areas and impacting important habitats. But, when multiple stressors act simultaneously, their effects on ecosystems become more difficult to predict. In Chesapeake Bay, USA, predictions suggest that anthropogenic stressors from climate change, such as warming temperatures, may increase the frequency and severity of storm events, leading to increased freshwater, nutrient, and sediment inputs. Coastal development, another source of anthropogenic stressors, continues to increase with growing coastal populations, and may lead to altered sediment characteristics, habitat fragmentation, altered food webs, and loss of vegetated habitats. Community processes may interact with stressors, for example, …


A Case Study In The Effectiveness Of Marine Protected Areas (Mpas): The Islands Of Bonaire And Curacao, Dutch Caribbean, Noelle J. Relles Jan 2012

A Case Study In The Effectiveness Of Marine Protected Areas (Mpas): The Islands Of Bonaire And Curacao, Dutch Caribbean, Noelle J. Relles

Dissertations, Theses, and Masters Projects

The islands of Bonaire and Curacao, Dutch Caribbean, were both mapped along their leeward coasts for dominant coral community and other benthic cover in the early 1980s. This mapping effort offers a unique baseline for comparing changes in the benthic community of the two islands since that time, particularly given the marked differences between the two islands. Bonaire is well-protected and completely surrounded by a marine protected area (MPA), which includes two no-diving marine reserves; additionally, Bonaire's population is only around 15,000. In contrast, the island of Curacao is home to 140,000 inhabitants and marine protection is limited, with a …


Unprecedented Restoration Of A Native Oyster Metapopulation, David M. Schulte Jan 2012

Unprecedented Restoration Of A Native Oyster Metapopulation, David M. Schulte

Dissertations, Theses, and Masters Projects

No abstract provided.


Copepod Carcasses, Mortality And Population Dynamics In The Tributaries Of The Lower Chesapeake Bay, David Thomas. Elliott Jan 2010

Copepod Carcasses, Mortality And Population Dynamics In The Tributaries Of The Lower Chesapeake Bay, David Thomas. Elliott

Dissertations, Theses, and Masters Projects

Several studies have documented the occurrence of substantial numbers of zooplankton carcasses in marine field samples. However, the potential effect of carcasses on conclusions resting on zooplankton abundance estimates, and the reasons for carcass occurrence have been largely disregarded. Many field studies do not account for the presence of carcasses in their sampling methodology. Zooplankton carcasses in situ are significant for several reasons. as concentrated particles of organic matter in the water column, zooplankton carcasses can be important vehicles for organic matter transport and hotspots of microbial abundance and activity. If dead animals are treated alive, carcasses could bias the …


A Numerical Modeling Study On Barotropic And Baroclinic Responses Of The Chesapeake Bay To Hurricane Events, Kyoung-Ho Cho Jan 2009

A Numerical Modeling Study On Barotropic And Baroclinic Responses Of The Chesapeake Bay To Hurricane Events, Kyoung-Ho Cho

Dissertations, Theses, and Masters Projects

The barotropic and baroclinic responses of the Chesapeake Bay to forcings from two hurricanes were investigated by using unstructured-grid three-dimensional hydrodynamic models. The model domain includes Chesapeake Bay proper, the tributaries, and its extended continental shelf in the mid-Atlantic Bight. Two hurricanes were studied: Hurricane Floyd of September, 1999 and Hurricane Isabel of September, 2003, both of which made landfall within 100 km of the Chesapeake Bay mouth. Hurricane Floyd in 1999 passed through the entrance of the Bay from southwest to northeast along the coastlines of Virginia as a Category 1 storm, whereas Hurricane Isabel in 2003 made landfall …


Effects Of Shoreline Development And Oyster Reefs On Benthic Communities In Lynnhaven, Virginia, Amanda Sue Lawless Jan 2008

Effects Of Shoreline Development And Oyster Reefs On Benthic Communities In Lynnhaven, Virginia, Amanda Sue Lawless

Dissertations, Theses, and Masters Projects

Shoreline hardening and construction of restoration oyster reefs are occurring at rapid rates throughout Chesapeake Bay and little research has been conducted to determine whether installment of a hardened shoreline and oyster reef placement has an effect on the surrounding benthic infaunal communities. I investigated the effects of shoreline development and oyster reefs on benthic communities in Lynnhaven, Virginia. Throughout Lynnhaven, I determined the effects of shoreline type (natural marsh, oyster reef, rip-rap and bulkhead), sediment grain size, Total Organic Carbon/Total Nitrogen (TOC/TN) of the sediment, and predation (caging study) on density, biomass, and diversity of benthic infauna. An information-theoretic …


Hypoxia And Macoma Balthica: Ecological Effects On A Key Infaunal Benthic Species, William Christopher Long Jan 2007

Hypoxia And Macoma Balthica: Ecological Effects On A Key Infaunal Benthic Species, William Christopher Long

Dissertations, Theses, and Masters Projects

Hypoxia, low dissolved oxygen, is an important environmental stressor in estuarine systems. In this dissertation, I examine the effects of hypoxia on the macrobenthic communities of the York and Rappahannock Rivers, Chesapeake Bay, USA, and in particular its effects on the infaunal clam Macoma balthica. A survey of the macrobenthic community was performed using box-coring before and after hypoxia in 2003 and 2004 in both rivers. Hypoxia was associated with a change in the macrobenthic community towards smaller, shorter-lived, opportunistic species; and a substantial decrease in biomass. M. balthica recruited into all areas of the river but suffered local extinction …


Development Of An Unstructured Grid, Finite Volume Eutrophication Model For The Shallow Water Coastal Bay: Application In The Lynnhaven River Inlet System, Yuepeng Li Jan 2006

Development Of An Unstructured Grid, Finite Volume Eutrophication Model For The Shallow Water Coastal Bay: Application In The Lynnhaven River Inlet System, Yuepeng Li

Dissertations, Theses, and Masters Projects

The shallow water region is an important portion of the estuarine and coastal waters, since it encompasses the entire land-water margin as the buffer zone and supports one of the most productive ecosystems. When light can penetrate to the sediment, it triggers the benthic microalgae community to perform photosynthesis, resulting in a benthic-pelagic exchange flux different from that of the deeper water. This study utilized the laboratory-measured benthic flux, and a suite of well-calibrated numerical models to examine the eutrophication process in the Lynnhaven River Inlet system with special emphasis on: the role played by benthic microalgae, and nutrient budgets …


The Effects Of Climate Change On The Population Ecology Of The Atlantic Surf Clam, Spisula Solidissima, In The Middle Atlantic Bight, Adriana Picariello Jan 2006

The Effects Of Climate Change On The Population Ecology Of The Atlantic Surf Clam, Spisula Solidissima, In The Middle Atlantic Bight, Adriana Picariello

Dissertations, Theses, and Masters Projects

No abstract provided.


Meiofauna Abundance And Distribution In Chesapeake Bay: Relationships With Environmental Stressors, Sediment Toxicity And Macrofauna, William J. Metcalfe Jan 2005

Meiofauna Abundance And Distribution In Chesapeake Bay: Relationships With Environmental Stressors, Sediment Toxicity And Macrofauna, William J. Metcalfe

Dissertations, Theses, and Masters Projects

Macrofauna-based biocriteria to assess impairment in aquatic communities are well-developed and have been widely accepted as useful for coastal monitoring programs worldwide. Meiofauna-based methods are not as well developed, but meiofauna are intimately associated with sediments through their life cycles and are functionally important. Thus, an understanding of meiofauna relationships with environmental quality is also important. Relationships between the abundance and composition of major meiofauna taxa for two shallow water habitat types (protected, with muddy sediment; exposed, with sandy sediment) were investigated along gradients associated with changing land use, sediment contamination and environmental stressors in Chesapeake Bay. Principal component analysis …


A Study Of The Eastern Oyster, Crassostrea Virginica: (1) Dermo Tolerance, Survival, Growth, Condition And Hsp70 Expression In Different Geographic Stocks; (2) Heat Tolerance And Effects Of Sublethal Heat Shock On Survival And Hsp70 Expression Of Infected And Uninfected Oysters, Vincent G. Encomio Jan 2004

A Study Of The Eastern Oyster, Crassostrea Virginica: (1) Dermo Tolerance, Survival, Growth, Condition And Hsp70 Expression In Different Geographic Stocks; (2) Heat Tolerance And Effects Of Sublethal Heat Shock On Survival And Hsp70 Expression Of Infected And Uninfected Oysters, Vincent G. Encomio

Dissertations, Theses, and Masters Projects

The diseases Dermo and MSX have devastated Chesapeake Bay populations of the eastern oyster, Crassostrea virginica. The protozoan Perkinsus marinus, which causes Dermo, is particularly problematic since it persists over a wide range of salinities and temperatures. An objective of this dissertation was to determine whether specific wild oyster stocks had developed natural resistance to Dermo and if several parameters (survival, growth, condition and energy reserves) were associated with resistance. Another objective was to characterize heat shock protein (hsp70) expression in the eastern oyster. Heat shock proteins such as hsp70 protect organisms from thermal stress and other stressors, and this …


Natural Dermo Resistance And Related Defense Mechanisms In Eastern Oysters, Crassostrea Virginica, With Implications For Restoration, Shawn Michael Stickler Jan 2004

Natural Dermo Resistance And Related Defense Mechanisms In Eastern Oysters, Crassostrea Virginica, With Implications For Restoration, Shawn Michael Stickler

Dissertations, Theses, and Masters Projects

American oyster (Crassostrea virginica) stocks on the East Coast in general, and in Chesapeake Bay in particular, have been in decline over the past half-century in large part due to Dermo disease, caused by the protist parasite Perkinsus marinus. Efforts to restore oysters for their ecological and economic value have centered on the development and use of hatchery-based seed selectively bred for disease resistance. Selective breeding could benefit from the incorporation of oysters from wild stocks that have developed "natural Dermo resistance", but few such stocks have been identified and verified. This dissertation describes reciprocal common-garden experiments carried out at …


Metal Concentrations In Deep-Sea Mussels From A Hydrothermal Vent And Cold Seep, Shana Rapoport Jan 2003

Metal Concentrations In Deep-Sea Mussels From A Hydrothermal Vent And Cold Seep, Shana Rapoport

Dissertations, Theses, and Masters Projects

No abstract provided.


Persistent Organic Pollutants At The Base Of The Antarctic Marine Food Web, Amy Lee Chiuchiolo Jan 2003

Persistent Organic Pollutants At The Base Of The Antarctic Marine Food Web, Amy Lee Chiuchiolo

Dissertations, Theses, and Masters Projects

No abstract provided.


Bioavailability Of Polybrominated Diphenyl Ethers (Pbdes) In Biosolids And Spiked Sediment To The Aquatic Worm Lumbriculus Variegatus, Serena Ciparis Jan 2003

Bioavailability Of Polybrominated Diphenyl Ethers (Pbdes) In Biosolids And Spiked Sediment To The Aquatic Worm Lumbriculus Variegatus, Serena Ciparis

Dissertations, Theses, and Masters Projects

No abstract provided.


Effects Of Urbanization And Land Use On Pah Accumulation In Wetland Sediments, Kimani L. Kimbrough Jan 2002

Effects Of Urbanization And Land Use On Pah Accumulation In Wetland Sediments, Kimani L. Kimbrough

Dissertations, Theses, and Masters Projects

The relationship between wetland polycyclic aromatic hydrocarbon (PAH) concentrations and adjacent land use was assessed using surface sediments, sediment cores and global information systems (GIS) in the Elizabeth River, Virginia, an urbanized subestuary of the Chesapeake Bay. Wetlands adjacent to parking lots and petroleum industrial sites exhibited the highest PAH concentrations of all land uses in surfaces sediment and historically. Automobile and coal are the dominant sources of PAHs in wetland sediments historically as determined by relative concentration, fingerprinting and source specific compounds. An analysis of variance (ANOVA) test used to compare sixteen surface sediment sites based on land use …


Fate Of Organic Compounds Associated With Extractable And Bound Phases Of Estuarine Sediments Deposited Under Varying Depositional Regimes, Krisa Murray Arzayus Jan 2002

Fate Of Organic Compounds Associated With Extractable And Bound Phases Of Estuarine Sediments Deposited Under Varying Depositional Regimes, Krisa Murray Arzayus

Dissertations, Theses, and Masters Projects

Surficial sediments and sediment cores were collected from two distinct depositional regimes of the York River subestuary of Chesapeake Bay to document seasonal inputs, spatial variability, and longer-term (>40 years) fate of total organic carbon (TOC), lipid biomarker compounds and polycyclic aromatic hydrocarbons (PAHs). These regimes included biological mixing in the lower York and episodic mixing at the mid river site. Compounds were selected to represent a range of chemical reactivities, biological and anthropogenic sources, and modes of entry to the environment. The depositional environments were characterized with a suite of analytical tools: x-radiographs, Eh, 210Pb and 137Cs, total …


Persistent Organic Pollutant Transport And Fate: Assessment By Molecular Tracers, Padma T. Venkatraman Jan 2001

Persistent Organic Pollutant Transport And Fate: Assessment By Molecular Tracers, Padma T. Venkatraman

Dissertations, Theses, and Masters Projects

Persistent organic pollutants (POPs) such as the organochlorine pesticide hexachlorocyclohexane (HCH) may undergo atmospheric transport and accumulate in regions remote from the source. It is important to develop techniques to help apportion source and identify transport or transformation processes to which HCHs and other mobile POPs may be subjected. Molecular tracers such as compound specific stable isotope and enantiomer ratios (ERs) may prove valuable in studying POP fate and transport. The objective of this study was to further develop the use of these two novel geochemical tools to evaluate the sources, transport and environmental fate of POPs, in the context …


Spatial And Temporal Distributions Of Organic Matter And Polycyclic Aromatic Hydrocarbons (Pahs) In Surface Waters Of The York River, Va Estuary, Rebecca E. Countway Jan 1999

Spatial And Temporal Distributions Of Organic Matter And Polycyclic Aromatic Hydrocarbons (Pahs) In Surface Waters Of The York River, Va Estuary, Rebecca E. Countway

Dissertations, Theses, and Masters Projects

No abstract provided.


Environmental Stress In Hard Coral: Evaluating Lipid As An Indicator Of Sublethal Stress On Short Time Scales, David Harold Niebuhr Jan 1999

Environmental Stress In Hard Coral: Evaluating Lipid As An Indicator Of Sublethal Stress On Short Time Scales, David Harold Niebuhr

Dissertations, Theses, and Masters Projects

Lipid quality was evaluated in Montastrea spp. under sediment- and heat-stressed conditions to evaluate lipid ratio as an indicator of sub-lethal stress on short time scales. The ratio of storage lipid (wax ester + triacylglyceride) to structural lipid (sterol esters + phospholipid) decreased significantly (0.25 to 0.14, p < 0.01) after experimental sedimentation. FAME analysis of colonies exposed to experimental sedimentation showed a reduction of the algal, 18:3(n-6) and 18:4(n-3), polyunsaturated fatty acid (PUFA) in the polar lipid fraction. This loss of PUFA suggests a loss of algal membrane in sediment-stressed colonies. Lipid quality was similarly measured in M. faveolata over a 10-day period. Mean (n = 20) ratio of storage to structural lipid in M. faveolata dropped from 2.43 to a level of 0.98 immediately following a natural sedimentation event before recovering to levels of 1.4 and 2.9 on post-storm days 2 and 4, respectively. Colonies of M. annularis subject to heat-stress (35??C) exhibited no significant change in storage lipid ratio, while levels of Free Fatty Acids increased significantly from 0.012 (n = 22) to 0.156 mg lipid/g dry tissue (n = 22)(p < 0.05). FAME analysis of tissue lipids extracted from the heat-stressed colonies showed changes in the polar fraction, with significant decreases in the 18:3(n-6), 18:3(n-3), 18:4(n-3), 20:4(n-6) and 20:5(n-3) (p < 0.05) PUFA and subsequent significant increases in the saturated fatty acids, 16:0 and 18:0 (p < 0.05). These changes in lipid quantity and quality indicate possible oxidation and preferential digestion of zooxanthellar membranes. Stress experiments were repeated in M. annularis using VacutainerRTM blood collection tubes to collect micro-tissue samples without destroying skeleton of the sample colonies. A significant decrease in storage: structural lipid ratio after sedimentation was also detected using the micro-tissue technique. This study indicates that the relative abundance of lipid subclass components can indicate sub-lethal environmental stress, on short time scales, in M. annularis and M. faveolata. Furthermore, micro-tissue collection techniques permit repeated monitoring coral colonies to assess the manifestation of stress from first detection of impact at the cellular level to changes in community to changes in community structure detectable over longer time scales.


Demersal Predator Exposure To Toxic Organic Contaminants: Direct Effects Of Macrofauna In Trophic Transfer, Patrick Winfield Lay Jan 1996

Demersal Predator Exposure To Toxic Organic Contaminants: Direct Effects Of Macrofauna In Trophic Transfer, Patrick Winfield Lay

Dissertations, Theses, and Masters Projects

The bioaccumulation and metabolism of organic contaminants (PAH, PCB) by three estuarine polychaetes, Nereis succinea (Frey and Leuckart 1847), Paraprionospio pinnata (Ehlers 1901) and Polydora ligni (Webster 1879), and by the fish predator, spot (Leiostomus xanthurus Lacepede 1802), were examined in laboratory experiments. Variations in trophic transfer of these contaminants resulting from differences in prey and contaminant type were also investigated. Toxicokinetic modeling reveals that metabolism of organic contaminants by invertebrate species result in variations in uptake, metabolism and elimination rate constants. Incorporation of prey metabolism potential in kinetic models increases the latter's predictability of biota-sediment accumulation factors (BSAFs), or …