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Molecular Characterization Of Soil Ammonia-Oxidizing Bacteria Based On The Genes Encoding Ammonia Monooxygenase, Jose Javier Alzerreca
Molecular Characterization Of Soil Ammonia-Oxidizing Bacteria Based On The Genes Encoding Ammonia Monooxygenase, Jose Javier Alzerreca
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Ammonia-oxidizing bacteria (AOB) are chemolithotrophs that oxidize ammonia/ammonium to nitrite in a two-step process to obtain energy for survival. AOB are difficult to isolate from the environment and iso lated strains may not represent the diversity in soil. A genetic database and molecular tools were developed based on the ammonia monooxygenase (AMO) encoding genes that can be used to assess the diversity of AOB that exist in soil and aquatic environments without the isolation of pure cultures. The amo genes have excellent potential as molecular markers; since AMO is only found in the AOB and is essential for their metabolism, …