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Full-Text Articles in Earth Sciences
Fate Of Stable Isotope Label During Predation Of 15N-Tagged Wild-Type Escherichia Coli By Protozoa, Ashley M. Barton
Fate Of Stable Isotope Label During Predation Of 15N-Tagged Wild-Type Escherichia Coli By Protozoa, Ashley M. Barton
University of Kentucky Master's Theses
Currently, bacterial movement in karst aquifers is not well understood. Use of stable isotopes to label non-pathogenic Escherichia coli as a particulate groundwater tracer in karst systems has been examined in previous studies. Loss of the stable isotope signal is anticipated in traces greater than 500 m in length. Potential loss of 15N due to predation by protozoa was examined. Filter-sterilized water from Royal Spring in Georgetown, Kentucky, was inoculated with a mixture of either Tetrahymena pyriformis or Colpoda steinii and 15N-enriched E. coli and stored in the dark at 14°C. Samples were analyzed for their nitrogen isotope …
Feasibility Of Using 15N-Enriched Escherichia Coli As A Bacterial Tracer In The Cane Run/Royal Spring Basin, Kentucky, John G. Warden
Feasibility Of Using 15N-Enriched Escherichia Coli As A Bacterial Tracer In The Cane Run/Royal Spring Basin, Kentucky, John G. Warden
University of Kentucky Master's Theses
A novel tracer method has used 15N to label Escherichia coli and track the transport of bacteria, a common contaminant, through karst aquifers. Use of this method could provide valuable insight into the movement of bacteria in aquifers, which would help improve remediation methods and strategies. A wild strain of E. coli was isolated from the Cane Run/Royal Spring basin in the Inner Bluegrass region of Kentucky. The strain was serotyped O-:H- and virulence testing showed the strain did not have virulence factors of E. coli commonly pathogenic to humans. Five karst microcosms were filled with …