Earth Sciences Commons^™

Tracing Nutrient Inputs Into A Eutrophic Lake Using Nitrogen Isotopes, Wilgreen Lake, Madison County, Kentucky, Walter S. Borowski, Theresa A. Aguiar

EKU Faculty and Staff Scholarship

Wilgreen Lake is a dammed lake that has been classified as nutrient-impaired (303d list) by the EPA and State of Kentucky. The lake is moderately-sized covering 169 acres (0.7 km²), and drains a watershed with residential developments, cattle pasture, modified woodlands, and some industrial/urban usage in the city of Richmond. The principal tributaries are Taylor Fork and Old Town Branch that meet to form the trunk of the lake approximately one mile in length. The upper reaches of Taylor Fork are adjacent to a densely-packed (quarter-acre lots) housing development with septic systems. Old Town Branch drains cattle pasture …

Using E. Coli And Bacteroides Distribution And Abundance In A Eutrophic Lake As A Tracer For Nutrient Inputs, Wilgreen Lake, Madison County, Kentucky, Theresa A. Aguiar, Walter S. Borowski, Alice C. Layton, Larry Mckay

EKU Faculty and Staff Scholarship

Wilgreen Lake is a eutrophic lake that has been listed on the EPA’s 303d list as nutrient impaired. Potential sources of this impairment are likely from humans, cattle manure, and fertilizers. We suspect that the majority of nutrients originate from human sources, namely from septic tank effluent emanating from key housing developments ringing the lakeshore. We test our hypothesis with conventional microbial assays (Escherichia coli) and RT-PCR techniques (Bacteroides).

We took water samples at 19 sampling locations on 4 occasions, and measured the abundance of Escherichia coli using IDEXX methods. Corresponding sub-samples slated for potential PCR …

Seasonal Changes In Stratification And Oxygen Content Of A Eutrophic Lake, Wilgreen Lake, Madison County, Kentucky, Jill Hunter, Walter S. Borowski

EKU Faculty and Staff Scholarship

Wilgreen Lake (Madison County, Kentucky) is listed by the Environmental Protection Agency as nutrient-impaired. The overabundance of nutrients is likely linked to the land-use practices in this area. Cattle pasture, residential developments served by septic systems, and urban/industrial areas lie in the lake’s watershed. We have studied the lake for two years to characterize its physical and chemical characteristics, and to identify nutrient sources.

The 2007 field season began in March and continued through October. We measured temperature and oxygen levels along with other parameters at 1-meter depth intervals at 19 stations distributed along the length of the lake and …