Chemistry Commons^™

Evaluation And In Vitro Studies Of Folate Peg Biotin And Other Peg Agents, Vikram Reddy Kasarala

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Folate is an essential component for cell growth and cell division, playing a major role in DNA synthesis. It is common sense that the rapidly dividing cells synthesize more DNA, including cancer cells. Also, it was reported that cancer cells over express Folate receptors and this was the basis for our probe for cancer cells. We used long PEG biotin in conjugation with Folate. Previous studies in our group showed that the PEG could be used in the sensor to detect and capture cancer cells. The present study was aimed at evaluating the effect of Long PEG containing Folate based …

Synthesis Of A Folate Receptor Mass Spectrometry Probe, Audrea Rhymes

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Drug delivery is one of the most important concepts for cancer patients because it is oftentimes very difficult or nearly impossible to exclusively target cancerous cells. In this study, the aim was to target receptors on cancerous cells, specifically the Folate Receptor (FR). This specific receptor was targeted because it has been proven to enhance intracellular delivery being that folic acid is an integral species in normal cell function and also that cancerous cells tend to exhibit an abnormally high concentration of Folic Acid on cell surfaces.

The application of dyes is a very common practice used to link folic …

Synthesis And Analytical Evaluation Of Folate Conjugates For Use In Cancer Cell Detection, Rakeshkumar V. Desai

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Folate receptors have two glycosyl phosphatidylinositol anchored isomers, alpha and beta. Folate receptor alpha binds with high affinity for folic acid and act as a receptor for mediated transport of folate into the cells. Folate is necessary for DNA metabolism and thus it is speculated that rapidly dividing cancer cells have an increased necessity for folic acid. Folate receptor alpha levels eminent in specific malignant diseases (like solid tumors and leukemia) and thus folate receptors serve in the detection of FR+ (Folate receptor positive) and diagnosis of cancers.

In general, liposomes have the capacity intake the most imaging and toxic …

Detection Of Folate Receptor From Fr+ Cancer Cells, Darpan Patel

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The aim was to detect cancer cells by using folate–PEG-Biotin probe with fluorescently labeled streptavidin for targeted drug delivery of anti-cancer drugs and diagnose cancer cells. Folate-PEG-Biotin (synthesized by Dr. Henne and Mr.Rohan Patel) was purified by HighPeformanceLiquidChromatography (HPLC) analysis and with the help of Liquid Chromatography/Mass Spectrometry (LC/MS) its identity was confirmed. Folate is a basic composition of cell metabolism in both synthesis of DNA and proteins. Growing cancer cells require high level of folic acid. Folate Receptor-α (FR-α) is a membrane bound protein having high affinity for folic acid and serves to transport of folate into cells. Poly …