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Full-Text Articles in Chemistry
Acrosome Reaction Is Subfamily Specific In Sea Star Fertilization, Mia Nakachi, Hideaki Moriyama, Motonori Hoshi, Midori Matsumoto
Acrosome Reaction Is Subfamily Specific In Sea Star Fertilization, Mia Nakachi, Hideaki Moriyama, Motonori Hoshi, Midori Matsumoto
Hideaki Moriyama Publications
In the fertilization process of sea stars, sperm is activated to go through the acrosome reaction before cell fusion. We focused on induction of the acrosome reaction as a key process in fertilization. Six species of sea stars were used in this study: Asterias amurensis, Asterias rubens, Asterias forbesi, Aphelasterias japonica, Distolasterias nipon, and Asterina pectinifera. Acrosome reaction assays indicate that the acrosome reaction can be induced across species within Asteriinae subfamily. However, cross-fertilization assays indicate that sea stars have species specificity in fertilization. Therefore, steps after the acrosome reaction are responsible for the …
The Importance Of The Strictly Conserved, C-Terminal Glycine Residue In Phosphoenolpyruvate Carboxylase For Overall Catalysis: Mutagenesis And Truncation Of Gly-961 In The Sorghum C4 Leaf Isoform, Wenxin Xu, Shaheen Ahmed, Hideaki Moriyama, Raymond Chollet
The Importance Of The Strictly Conserved, C-Terminal Glycine Residue In Phosphoenolpyruvate Carboxylase For Overall Catalysis: Mutagenesis And Truncation Of Gly-961 In The Sorghum C4 Leaf Isoform, Wenxin Xu, Shaheen Ahmed, Hideaki Moriyama, Raymond Chollet
Hideaki Moriyama Publications
Phosphoenolpyruvate carboxylase (PEPC) is a “multifaceted,” allosteric enzyme involved in C4 acid metabolism in green plants/microalgae and prokaryotes. Before the elucidation of the three-dimensional structures of maize C4 leaf and Escherichia coli PEPC, our truncation analysis of the sorghum C4 homologue revealed important roles for the enzyme’s C-terminal α-helix and its appended QNTG961 tetrapeptide in polypeptide stability and overall catalysis, respectively. Collectively, these functional and structural observations implicate the importance of the PEPC C-terminal tetrapeptide for both catalysis and negative allosteric regulation. We have now more finely dissected this element of PEPC structure-function by modification of the absolutely conserved …