Chemistry Commons^™

Chlorella Virus-Encoded Deoxyuridine Triphosphatases Exhibit Different Temperature Optima, Yuanzheng Zhang, Hideaki Moriyama, Kohei Homma, James L. Van Etten

Hideaki Moriyama Publications

A putative deoxyuridine triphosphatase (dUTPase) gene from chlorella virus PBCV-1 was cloned, and the recombinant protein was expressed in Escherichia coli. The recombinant protein has dUTPase activity and requires Mg²⁺ for optimal activity, while it retains some activity in the presence of other divalent cations. Kinetic studies of the enzyme revealed a K_m of 11.7 μ M, a turnover k_cat of 6.8 s^-1, and a catalytic efficiency of k_cat/K_m = 5.8 x 10⁵ M^-1 s^-1. dUTPase genes were cloned and expressed from two other chlorella viruses …