Chemistry Commons^™

Metallopeptides From Design To Catalysis: Structure, Oxidative Activities, And Inhibition Studies Of Designed And Naturally Occurring Metallopeptides, Alaa Hassan Hashim

USF Tampa Graduate Theses and Dissertations

Structural and mechanistic complexities of copper-dioxygen systems have attracted much attention in the field of bioinorganic chemistry, both in model systems and trapped protein intermediates. The research presented herein is focused on model and naturally occurring metallopeptide systems, from its design to catalysis. Copper is used as the coordinating metal ion, with cobalt and zinc as probes for metal binding. The bioinorganic chemistry of copper proteins and its coordination and spectroscopic properties are briefly discussed in chapter 1. The next two chapters are centered on the de novo design of a minimalistic metallopeptide system with an amino acid sequence of …

Mechanistic Studies Of Salt Effects On Bimolecular Electron-Transfer Reactions Of Pentaamine Ruthenium Pyridyl Complexes Studied By 19f Nmr Line-Broadening And T2 Spin-Echo Techniques, Nicholas J. Magarian

Master's Theses

Kinetic salt effects on the bimolecular ET self-exchange reaction between pentaamineruthenium(II)(3-trifluoromethylpyridine)²⁺, (NH₃)₅Ru^IItfmp²⁺, and pentaamineruthenium(III)(3-trifluoromethylpyridine)³⁺, (NH₃)₅Ru^IIItfmp³⁺, have been measured using both ¹⁹F NMR line-broadening and CPMG T₂ spin-echo relaxation techniques in H₂O and D₂O. Over the equimolar reactants concentration range of 0.10 mM – 8.00 mM there was a definite “self-salting” rate increase arising from the increased solution ionic strengths due to the reactants and counterions themselves. The magnitude of this effect diverged significantly, however, …

Structural Studies Of Bile Salt Aggregation By Nuclear Magnetic Resonance Spectroscopy And Mass Spectrometry, Nicholas Doyle

Master’s Theses

Bile salts are biomolecules that are produced in the liver and are responsible for a range of functions in the process of digestion, primarily the emulsification of dietary fat and fat-soluble vitamins. Despite their importance in biological chemistry, the structure and dynamics of bile salt aggregation are not well understood. The efforts described herein attempt to enhance the understanding of cholate aggregation numbers (AN), critical micelle concentration (CMC), micellar structure(s), and interactions with a binaphthyl probe molecule. Cholate is the most common bile salt in mammals and is, therefore, a decent model for describing bile salt aggregation. CMC determination is …

Effects Of Statin Drugs And Tocotrienol Rich Fraction Supplementation In Chronic Hemodialysis Patients And Metabolomic Profile, Eno Latifi

Wayne State University Theses

Chronic kidney disease (CKD) is known as a heterogeneous disorder which currently is on the rise and lately has been classified as a public health issues in the United State and worldwide. CKD is an irreversible and progressive disease which can lead to kidney failure, and this is depicted by the advanced stage of the disorder when it reaches the point, that is classified as end stage of renal disease (ESRD) (Stage 5 of CKD) (eGRF <15 mL/min/ 1.73 m2 working capacity), where both organs are in a total or permanent kidney failure. End-Stage renal disease patients, on hemodialysis have been associated to experience an accelerated form of atherosclerosis, which is induced by inflammation, impairment of antioxidant system and elevated oxidative stress. Since the problem effecting ESRD patients is multifactorial, the objective of this investigation is to explore and look at the effects of supplementing with vitamin E-tocotrienol rich fraction (TRF), a micronutrient which has anti-inflammatory, antioxidant, and lipid lower capabilities into tackling these comorbid conditions experienced by this population. Therefore the aims of this investigation will be to explore changes in lipid profiles, inflammatory markers, and oxidative status, as well as look at any changes in metabolomic profiles. It was hypothesized that by supplementing with TRF a vitamin E, for 16 weeks in ESRD patients undergoing hemodialysis, it may help reverse and/or improve, oxidative status, inflammatory markers, increase antioxidants status and improve lipid profiles.

The study was double-blinded, randomized, parallel, placebo-controlled design trial, of 81 adult patients undergoing chronic hemodialysis at Great Lake Dialysis Clinic, Detroit MI, where …

Spectroscopic Characterization Of Dissolved Organic Matter: Insights Into The Linkage Between Sources And Chemical Composition, Xiaoyan Cao

Chemistry & Biochemistry Theses & Dissertations

This dissertation investigated the chemical structure of DOM by advanced solid-state nuclear magnetic resonance (NMR) spectroscopy and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) techniques, as well as isotopic measurements and UV-visible spectroscopy, to shed light on the linkages between DOM sources and DOM composition. Unique and extensive sets of DOM samples studied here were isolated from various aquatic systems, covering end-member environments in which DOM is considered either microbially derived or terrestrially derived, and areas in which DOM has characteristics intermediate between the two end members. Important insights into specific site-related questions were also gained such as …