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Full-Text Articles in Chemistry
Development Of Mass Spectrometry-Based Methods For Quantitation And Characterization Of Protein Drugs: Transferrin As A Model Drug Delivery Vehicle, Shunhai Wang
Open Access Dissertations
In the last two decades, protein drugs have enjoyed a rapid growth and achieved a tremendous success in treating human diseases. However, the presence of physiological barriers greatly impedes the efficient delivery of such unconventional large molecule drugs, and therefore limits their clinical utility. An elegant way to address this challenge takes advantage of certain endogenous transporter proteins, such as human transferrin (Tf), whose ability to traverse physiological barriers has been extensively exploited. However, methods to investigate Tf-based drug delivery remained insufficient and unsatisfactory until recent development of quantitative mass spectrometry (MS). Hereby, MS-based methods have been developed and validated …
Structural Characterization Of The Pre-Amyloid Oligomers Of Β-2-Microglobulin Using Covalent Labeling And Mass Spectrometry, Vanessa Leah Castillo Mendoza
Structural Characterization Of The Pre-Amyloid Oligomers Of Β-2-Microglobulin Using Covalent Labeling And Mass Spectrometry, Vanessa Leah Castillo Mendoza
Open Access Dissertations
The initial steps involved in the assembly of normally soluble proteins into amyloid fibrils remain unclear, yet over 20 human diseases are associated with proteins that aggregate in this manner. Protein surface modification is a potential means of mapping the interaction sites in early oligomers that precede amyloid formation. This dissertation focuses on the use of covalent labeling combined with mass spectrometry to elucidate the structural features of Cu(II)-induced β-2-microglobulin (β2m) amyloid formation. An improved covalent modification and MS-based approach for protein surface mapping has been developed to address the need for a reliable approach that ensures protein structural integrity …
Developing Novel Electrospray Ionization Mass Spectrometry (Esi Ms) Techniques To Study Higher Order Structure And Interaction Of Biopolymers, Agya K. Frimpong
Developing Novel Electrospray Ionization Mass Spectrometry (Esi Ms) Techniques To Study Higher Order Structure And Interaction Of Biopolymers, Agya K. Frimpong
Open Access Dissertations
Mass spectrometry has enjoyed enormous popularity over the years for studying biological systems. The theme of this dissertation was to develop and use mass spectrometry based tools to solve five biologically oriented problems associated with protein architecture and extend the utility of these tools to study protein polymer conjugation. The first problem involved elucidating the false negatives of how proteins with few basic residues, forms highly charged ions in electrospray ionization mass spectrometry (ESI MS). This study showed that the unfolding of polypeptide chains in solution leads to the emergence of highly charged protein ions in ESI MS mass spectra, …