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Full-Text Articles in Chemistry
Infrared Laser Ablation For Biomolecule Sampling, Kelin Wang
Infrared Laser Ablation For Biomolecule Sampling, Kelin Wang
LSU Doctoral Dissertations
In this research, an infrared laser at a wavelength of 3 µm was used to ablate material from tissue sections for biomolecule analysis. Pulsed infrared (IR) irradiation of tissue with a focused laser beam efficiently removed biomolecules, such as proteins, enzymes, DNA, and RNA from tissue sections for further analysis. In a proteomics project, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was used to determine regions of interest (ROI) for laser ablation. The matrix was then washed off. By overlaying the MSI generated heat-map, the section was sampled using IR laser ablation and custom stage-control software. Two ROI were selected …
Nanoscale Studies Of Proteins And Thin Films Using Scanning Probe Microscopy, Zachary Lane Highland
Nanoscale Studies Of Proteins And Thin Films Using Scanning Probe Microscopy, Zachary Lane Highland
LSU Doctoral Dissertations
Nanostructures of organosilanes, thin metal films, and protein nanopatterns were prepared and analyzed with atomic force microscopy (AFM). Organosilanes with designed functional groups were used to selectively pattern green fluorescent protein at the nanoscale using protocols developed with particle lithography. Mesospheres are deposited onto a substrate to produce a surface mask. Organosilanes are deposited to form a matrix film surrounding nanopores for depositing proteins. The nanopatterns were characterized using AFM, after steps of particle lithography for directly visualizing surface changes. Studies with AFM also provide a compelling tool for teaching undergraduates to introduce concepts of nanoscience. An undergraduate laboratory was …
Bio-Imprinted Hydro-Gels (Bigs) For Protein And Virus Detection, Wei Bai
Bio-Imprinted Hydro-Gels (Bigs) For Protein And Virus Detection, Wei Bai
LSU Doctoral Dissertations
Detection of bio-markers at low concentration is becoming a more and more important topic in scientific research due to its importance in applications crucially related to people’s life like medical diagnosis, environment protection and national security. In the past decades, as the improvement of the modern analytical technologies progressed, plenty of methodologies have been developed to realize the fast and accurate detection of bio-markers in liquid media. However, some drawbacks still remain like the expensive cost, high requirement of operational environment, and need for skilled operators. Here, a new kind of aptamer-based bio-imprinted hydrogel sensor (BIG) with specific macroscopic volume …
Microfluidic Devices Interfaced To Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry For Proteomics, Jeonghoon Lee
Microfluidic Devices Interfaced To Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry For Proteomics, Jeonghoon Lee
LSU Doctoral Dissertations
Microfluidic interfaces were developed for off-line matrix-assisted laser desorption/ionization mass spectrometry (MALDI). Microfluidic interfaces allow samples to be manipulated on-chip and deposited onto a MALDI target plate for analysis. For this research, microfluidic culturing devices and automated digestion and deposition microfluidic chip platforms were developed for the identification of proteins. The microfluidic chip components were fabricated on a poly(methyl methacrylate), PMMA, wafer using the hot embossing method and a molding tool with structures prepared via micromilling. One of the most important components of the chip system was a trypsin microreactor. An open channel microreactor was constructed in a 100 µm …
Off-Line Maldi Mass Spectrometry Of Bioaerosols, Jae-Kuk Kim
Off-Line Maldi Mass Spectrometry Of Bioaerosols, Jae-Kuk Kim
LSU Doctoral Dissertations
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used for off-line analysis of bioaerosols. The overall goal of the work is to develop methods and instrumentation to minimize sample treatment and maximize sensitivity and selectivity for bioaerosol analysis. This work is divided into three parts: sample preparation, test bioaerosol analysis, and instrument development. Untreated silicon wafer substrates were used as an alternate to conventional stainless steel targets and proved to be excellent substrates for large molecule analyses as well as small molecule analyses with excellent spot-to-spot and shot-to-shot reproducibility, which is useful for high-throughput and automated sample analysis. MALDI sample preparation …
Simulating Protein Evolution Via Thermodynamic Models, Yanlong Xu
Simulating Protein Evolution Via Thermodynamic Models, Yanlong Xu
LSU Doctoral Dissertations
Natural proteins are results of evolution and they need to maintain certain thermodynamic stabilities in order to carry out their biological functions. By simulating protein evolution based on thermodynamic rules, we could reconstruct the evolution trajectory and analyze the evolutionary dynamics of a protein population, and further understand the protein sequence-structure-function relationship. In this study, we have used both a simplified lattice model and a high-resolution atomic model to simulate protein evolution processes. With the lattice model, we have investigated general theoretical questions about how protein structural designability would affect protein evolution, particularly how it would affect protein recombination and …