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Protein Engineering Of Cota Laccase By Using Bacillus Subtilis Spore Display, Silu Sheng
Protein Engineering Of Cota Laccase By Using Bacillus Subtilis Spore Display, Silu Sheng
Dissertations
Spore display offers advantages over more commonly utilized microbe cell-surface display systems. For instance, protein-folding problems associated with the expressed recombinant polypeptide crossing membranes are avoided. Hence, a different region of protein space can be explored that previously was not accessible. In addition, spores tolerate many physical/chemical extremes. The aim is to improve pH stability using spore display. The maximum activity of CotA is between pH 4 and 5 for the substrate ABTS (ABTS = diammonium 2, 2’-azino-bis(3-ethylbenzothiazoline-6-sulfonate)). However, the activity dramatically decreases at pH 4. The activity is not significantly altered at pH 5. CotA is used as a …