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Full-Text Articles in Chemistry
Two Forms Of Tyrosyl-Trna Synthetase From Pseudomonas Aeruginosa: Characterization And Discovery Of Inhibitory Compounds, Casey Anne Hughes, Varesh Gorabi, Yaritza Escamilla, Frank B. Dean, James M. Bullard
Two Forms Of Tyrosyl-Trna Synthetase From Pseudomonas Aeruginosa: Characterization And Discovery Of Inhibitory Compounds, Casey Anne Hughes, Varesh Gorabi, Yaritza Escamilla, Frank B. Dean, James M. Bullard
Chemistry Faculty Publications and Presentations
Pseudomonas aeruginosa is a multidrug-resistant (MDR) pathogen and a causative agent of both nosocomial and community-acquired infections. The genes (tyrS and tyrZ) encoding both forms of P. aeruginosa tyrosyl-tRNA synthetase (TyrRS-S and TyrRS-Z) were cloned and the resulting proteins purified. TyrRS-S and TyrRS-Z were kinetically evaluated and the Km values for interaction with Tyr, ATP, and tRNATyr were 172, 204, and 1.5 μM and 29, 496, and 1.9 μM, respectively. The kcatobs values for interaction with Tyr, ATP, and tRNATyr were calculated to be 3.8, 1.0, and 0.2 s–1 and 3.1, 3.8, and 1.9 s–1, …
Glutaminyl-Trna Synthetase From Pseudomonas Aeruginosa: Characterization, Structure, And Development As A Screening Platform, Yaritza Escamilla, Casey A. Hughes, Jan Abendroth, David M. Dranow, Samantha Balboa, Frank B. Dean, James M. Bullard
Glutaminyl-Trna Synthetase From Pseudomonas Aeruginosa: Characterization, Structure, And Development As A Screening Platform, Yaritza Escamilla, Casey A. Hughes, Jan Abendroth, David M. Dranow, Samantha Balboa, Frank B. Dean, James M. Bullard
Chemistry Faculty Publications and Presentations
Pseudomonas aeruginosa has a high potential for developing resistance to multiple antibiotics. The gene (glnS) encoding glutaminyl-tRNA synthetase (GlnRS) from P. aeruginosa was cloned and the resulting protein characterized. GlnRS was kinetically evaluated and the KM and kcatobs , governing interactions with tRNA, were 1.0 μM and 0.15 s-1 , respectively. The crystal structure of the α2 form of P. aeruginosa GlnRS was solved to 1.9 Å resolution. The amino acid sequence and structure of P. aeruginosa GlnRS were analyzed and compared to that of GlnRS from Escherichia coli. Amino acids that interact with ATP, glutamine, and tRNA are well …
Lysyl-Trna Synthetase From Pseudomonas Aeruginosa: Characterization And Identification Of Inhibitory Compounds, Samantha Balboa, Yanmei Hu, Frank B. Dean, James M. Bullard
Lysyl-Trna Synthetase From Pseudomonas Aeruginosa: Characterization And Identification Of Inhibitory Compounds, Samantha Balboa, Yanmei Hu, Frank B. Dean, James M. Bullard
Chemistry Faculty Publications and Presentations
Pseudomonas aeruginosa is an opportunistic pathogen that causes nosocomial infections and has highly developed systems for acquiring resistance against numerous antibiotics. The gene (lysS) encoding P. aeruginosa lysyl-tRNA synthetase (LysRS) was cloned and overexpressed, and the resulting protein was purified to 98% homogeneity. LysRS was kinetically evaluated, and the Km values for the interaction with lysine, adenosine triphosphate (ATP), and tRNALys were determined to be 45.5, 627, and 3.3 µM, respectively. The kcatobs values were calculated to be 13, 22.8, and 0.35 s−1, resulting in kcatobs/KM values of 0.29, 0.036, and 0.11 s−1µM−1, …