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Protein Synthesis In Rabbit Reticulocytes: Characteristics Of A Ribosomal Factor That Reverses Inhibition Of Protein Synthesis In Heme-Deficient Lysates, R. O. Ralston, A. Das, A. Dasgupta, R. Roy, S. Palmieri, N. K. Gupta
Protein Synthesis In Rabbit Reticulocytes: Characteristics Of A Ribosomal Factor That Reverses Inhibition Of Protein Synthesis In Heme-Deficient Lysates, R. O. Ralston, A. Das, A. Dasgupta, R. Roy, S. Palmieri, N. K. Gupta
Chemistry Department: Faculty Publications
A ribosomal salt (0.5 M KCI) wash factor (RF) that reverses inhibition of protein synthesis in heme-deficient reticulocyte lysates has been resolved from the bulk of Met-tRNAtmet- binding factor (EIF-1), Co-EIF-I, and EIF-2 (ternary complex dissociation factor, TDF). The purified RF restores protein synthesis activity of heme-deficient lysates to the level observed in the presence of hemin. No direct correlation exists between amount of EIF-i activity and ability to reverse inhibition of protein synthesis in heme-deficient lysates. Homogeneous preparations of EIF-i are completely inactive in reversal of protein synthesis inhibition in heme-deficient lysates. These findings suggest that …