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Auctus: The Journal of Undergraduate Research and Creative Scholarship
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Investigation Of Factors Affecting Reverse-Phase High Performance Liquid Chromatography, Rida A. Syed
Investigation Of Factors Affecting Reverse-Phase High Performance Liquid Chromatography, Rida A. Syed
Auctus: The Journal of Undergraduate Research and Creative Scholarship
This study focused on the application of reverse-phase high-performance liquid chromatography (RP-HPLC). RP-HPLC is used for the purification of organic molecules, including pharmaceuticals, natural products, and proteins. We developed a modified version of a United States Pharmacopeia (USP) procedure to separate and purify the chemical components found in Excedrin tablets. The goal was to investigate the relationship between retention time (Tr) and the capacity factor (k') of solutes. By varying the composition of the mobile phase and stationary phase, we experimentally determined the capacity factor of acetaminophen (APAP), caffeine, and aspirin. Our results show that the capacity factor (k') of …