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Full-Text Articles in Chemistry
Characterization Of A Novel Fe-S Cluster Transfer Pathway Between Grx4 And Sufa In Escherichia Coli, Enis Sanchez
Characterization Of A Novel Fe-S Cluster Transfer Pathway Between Grx4 And Sufa In Escherichia Coli, Enis Sanchez
Theses and Dissertations
Biogenesis of iron-sulfur (Fe-S) clusters is an essential process in living organisms due to the critical role of Fe-S cluster proteins in a myriad of cellular functions. During the assembly of Fe-S clusters, multi-protein complexes are used to drive the mobilization and protection of reactive sulfur and iron intermediates, regulate assembly of various Fe-S clusters on an ATPase-dependent, multi-protein scaffold, and target nascent clusters to their downstream protein targets. In each of the Fe-S cluster biogenesis steps, specific protein-protein interactions (PPIs) are required for proper function of the assembly pathway. The target pathway is the sulfur formation (Suf) pathway for …
Iron Transport Machinery: A Potential Therapeutic Target In Escherichia Coli, Clorissa L. Washington – Hughes
Iron Transport Machinery: A Potential Therapeutic Target In Escherichia Coli, Clorissa L. Washington – Hughes
Theses and Dissertations
Iron metabolism is an integral part of life for most organisms. Despite its essentiality, iron can also become toxic. There are many aspects to maintaining iron balance within the cell. The aim of this work is to provide insight into the function of several components involved in bacterial iron homeostasis. This work is significant for the development of novel antibiotics for treating resistant or pathogenic bacteria. Herein, it is shown that nickel can disrupt normal bacterial iron metabolism and that bacterial resistance can be affected by expression of iron acquisition genes. Once iron is obtained by the cell, it can …
Characterization Of Sufs And Sufe Of Suf Pathway For Fe-S Cluster Assembly In Escherichia Coli, Guangchao Dong
Characterization Of Sufs And Sufe Of Suf Pathway For Fe-S Cluster Assembly In Escherichia Coli, Guangchao Dong
Theses and Dissertations
Fe-S clusters are one major type of the sulfur-containing cofactors, which conduct essential functions in organisms. The Suf pathway is one of the three main pathways for the biosynthesis of Fe-S clusters. In E. coli, the Suf pathway is utilized under iron limitation and oxidative stress. This ability is important for pathogens to survive. Also, the Suf pathway is found to be exclusive in bacteria, so it is a good target for novel antibiotic design. SufS is a cysteine desulfurase in the Suf pathway to extract sulfur from L-cysteine. It needs the enhancement of SufE. To better understand the catalytic …
Characterization Of The Suf Fe-S Pathway In Escherichia Coli, Naimah Bolaji
Characterization Of The Suf Fe-S Pathway In Escherichia Coli, Naimah Bolaji
Theses and Dissertations
Iron is an essential transition metal required by almost all organisms for use as a cofactor in many metabolic processes such as respiration and photosynthesis. Iron can be combined with elemental sulfur to form an iron-sulfur (Fe-S) cluster. In bacterial pathogens, Fe-S cluster cofactors carry out critical functions and the Fe-S cluster biogenesis pathway is essential for their survival. In E. coli, the Suf pathway assembles Fe-S clusters under conditions of iron starvation and oxidative stress. While some mechanistic details of the Fe-S cluster biogenesis have been well-characterized, the process of in vivo iron donation remains unclear. Iron storage proteins …
Iron Uptake And Accumulation Is A Target Of Nickel Toxicity During The Lag Phase In Escherichia Coli, Geoffrey Tuttle Ford
Iron Uptake And Accumulation Is A Target Of Nickel Toxicity During The Lag Phase In Escherichia Coli, Geoffrey Tuttle Ford
Theses and Dissertations
Various transition metals are essential to all forms of life, and are only required in trace amounts. But this dependence comes as a double-edged sword. All organisms must maintain a careful intracellular quota that does not traverse outside an acceptable range. One transition metal in particular is nickel. The importance of this transition metal has been debated widely and its function varies greatly between organisms, including bacteria. However, the adverse effects caused by over exposure to this metal have been the center of much experimentation in recent years. Still, the mechanisms of nickel toxicity and the subsequent effects on cellular …