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Full-Text Articles in Chemistry
Staphylococcus Aureus Helicase But Not Escherichia Coli Helicase Stimulates S. Aureus Primase Activity And Maintains Initiation Specificity, Scott A. Koepsell, Marilynn A. Larson, Mark A. Griep, Steven H. Hinrichs
Staphylococcus Aureus Helicase But Not Escherichia Coli Helicase Stimulates S. Aureus Primase Activity And Maintains Initiation Specificity, Scott A. Koepsell, Marilynn A. Larson, Mark A. Griep, Steven H. Hinrichs
Mark Griep Publications
Bacterial primases are essential for DNA replication due to their role in polymerizing the formation of short RNA primers repeatedly on the lagging-strand template and at least once on the leading-strand template. The ability of recombinant Staphylococcus aureus DnaG primase to utilize different single-stranded DNA templates was tested using oligonucleotides of the sequence 5’-CAGA (CA) 5 XYZ (CA) 3-3’, where XYZ represented the variable trinucleotide. These experiments demonstrated that S. aureus primase synthesized RNA primers predominately on templates containing 5’-d(CTA)-3’ or TTA and to a much lesser degree on GTA-containing templates, in contrast to results seen with the Escherichia …
Kinetics Of The Dna Polymerase Pyrococcus Kodakaraensis, Mark A. Griep, Casey A. Kotera, R.M. Nelson, Hendrik J. Viljoen
Kinetics Of The Dna Polymerase Pyrococcus Kodakaraensis, Mark A. Griep, Casey A. Kotera, R.M. Nelson, Hendrik J. Viljoen
Mark Griep Publications
The polymerase chain reaction is one of the most important reactions in molecular biology. Single stranded DNA is copied in a complex series of steps, at the core of which lies the action of the DNA polymerase. At each nucleotide along the template, the polymerase screens the dNTP pool until it finds the complementary dNTP. The insertion of each dNMP is a balance between high fidelity and rapid elongation. In this study the kinetics of the β type polymerase pyrococcus kodakaraensis (KOD) is analyzed. The kinetics is influenced by reaction conditions such as the dNTP pool composition and temperature. In …