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Full-Text Articles in Chemistry
Functional Replacement Of The Ketosynthase Domain Of Fum1 For The Biosynthesis Of Fumonisins, A Group Of Fungal Reduced Polyketides, Xiangcheng Zhu, Fengan Yu, R. S. Bojja, K. Zaleta-Rivera, Liangcheng Du
Functional Replacement Of The Ketosynthase Domain Of Fum1 For The Biosynthesis Of Fumonisins, A Group Of Fungal Reduced Polyketides, Xiangcheng Zhu, Fengan Yu, R. S. Bojja, K. Zaleta-Rivera, Liangcheng Du
Liangcheng Du Publications
The genetic manipulation of the biosynthesis of fungal reduced polyketides has been challenging due to the lack of knowledge on the biosynthetic mechanism, the difficulties in the detection of the acyclic, non-aromatic metabolites, and the complexity in genetically manipulating filamentous fungi. Fumonisins are a group of economically important mycotoxins that contaminate maize-based food and feed products worldwide. Fumonisins contain a linear dimethylated C18 chain that is synthesized by Fum1p, which is a single module polyketide synthase (PKS). Using a genetic system that allows the specific manipulation of PKS domains in filamentous fungus Fusarium verticillioides, we replaced the KS domain …
Supplemental Materials For "Distinct Ceramide Synthases Regulate Polarized Growth In The Filamentous Fungus Aspergillus NidulansD", Shaojie Li, Liangcheng Du, Gary Yuen, Steven D. Harris
Supplemental Materials For "Distinct Ceramide Synthases Regulate Polarized Growth In The Filamentous Fungus Aspergillus NidulansD", Shaojie Li, Liangcheng Du, Gary Yuen, Steven D. Harris
Liangcheng Du Publications
Four figures not included in the print edition of the above-referenced article, that was published in Molecular Biology of the Cell, Vol. 17, 1218–1227 (March 2006). :
Figure 1 - barA mutants are resistant to the effects of HPLC-purified HSAF.
Figure 2 - Alignment of Lag1 homologues.
Figure 3 - Alignments of BasA homologues.
Figure 4 - Growth defects of lagA mutants.
Distinct Ceramide Synthases Regulate Polarized Growth In The Filamentous Fungus Aspergillus NidulansD, Shaojie Li, Liangcheng Du, Gary Yuen, Steven D. Harris
Distinct Ceramide Synthases Regulate Polarized Growth In The Filamentous Fungus Aspergillus NidulansD, Shaojie Li, Liangcheng Du, Gary Yuen, Steven D. Harris
Liangcheng Du Publications
In filamentous fungi, the stabilization of a polarity axis is likely to be a pivotal event underlying the emergence of a germ tube from a germinating spore. Recent results implicate the polarisome in this process and also suggest that it requires localized membrane organization. Here, we employ a chemical genetic approach to demonstrate that ceramide synthesis is necessary for the formation of a stable polarity axis in the model fungus Aspergillus nidulans. We demonstrate that a novel compound (HSAF) produced by a bacterial biocontrol agent disrupts polarized growth and leads to loss of membrane organization and formin localization at …