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University of Nebraska - Lincoln

Department of Chemistry: Dissertations, Theses, and Student Research

2006

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A Comparative Genomic Analysis Of Chlorella Nc64a Virus Ny-2a And Chlorella Pbi Virus Mt325 From The Family Phycodnaviridae, Lisa A. Fitzgerald Mar 2006

A Comparative Genomic Analysis Of Chlorella Nc64a Virus Ny-2a And Chlorella Pbi Virus Mt325 From The Family Phycodnaviridae, Lisa A. Fitzgerald

Department of Chemistry: Dissertations, Theses, and Student Research

The family Phycodnaviridae consists of a morphologically similar but genetically diverse group of large dsDNA viruses which infect both fresh and marine water eukaryotic algae. Two viruses, the 370 kb Chlorella NC64A virus NY-2A and the 313 kb Chlorella Pbi virus MT325, from the family Phycodnaviridae, genus Chlorovirus, were sequenced, analyzed, and compared to the prototype chlorella virus PBCV-1. The NY-2A genome, the largest chlorella viral genome sequenced to date, contains 886 open reading frames (ORFs) of 65 codons or larger and encodes 404 putative proteins and 7 tRNAs. The MT325 genome, the smallest chlorella viral genome sequenced to date, …