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Chromatographic Analysis Of Carbamazepine Binding To Human Serum Albumin: Ii. Comparison Of The Schiff Base And N-Hydroxysuccinimide Immobilization Methods, Hee Seung Kim, Rangan Mallik, David S. Hage
Chromatographic Analysis Of Carbamazepine Binding To Human Serum Albumin: Ii. Comparison Of The Schiff Base And N-Hydroxysuccinimide Immobilization Methods, Hee Seung Kim, Rangan Mallik, David S. Hage
David Hage Publications
Recent studies with carbamazepine on human serum albumin (HSA) columns have noted an appreciable degree of non-specific binding on supports prepared by the Schiff base immobilization method. This work examines an alternative immobilization method for HSA based on N-hydroxysuccinimide (NHS)-activated silica. This support was prepared by reacting HPLC-grade silica directly with disuccinimidyl carbonate. The resulting material was compared to an HSA support prepared by the Schiff base method in terms of its activity for carbamazepine and non-specific interactions with this drug. When examined by frontal analysis, both supports gave comparable association equilibrium constants for carbamazepine interactions with HSA ((0.53–0.55) …
Studies By Biointeraction Chromatography Of Binding By Phenytoin Metabolites To Human Serum Albumin, Corey M. Ohnmacht, Shirley Chen, Zenghan Tong, David S. Hage
Studies By Biointeraction Chromatography Of Binding By Phenytoin Metabolites To Human Serum Albumin, Corey M. Ohnmacht, Shirley Chen, Zenghan Tong, David S. Hage
David Hage Publications
Biointeraction studies based on high performance affinity chromatography were used to investigate the binding of human serum albumin (HSA) to two major phenytoin metabolites: 5-(3-hydroxyphenyl)-5-phenylhydantoin (m-HPPH) and 5-(4-hydroxyphenyl)-5-phenylhydantoin (p-HPPH). This was initially examined by conducting self-competition zonal elution experiments in which m-HPPH or p-HPPH were placed in both the mobile phase and injected sample. It was found that each metabolite had a single major binding site on HSA. Competitive zonal elution experiments using l-tryptophan, warfarin, digitoxin, and cis-clomiphene as site-selective probes indicated that m-HPPH and p-HPPH were interacting with the indolebenzodiazepine …