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Full-Text Articles in Chemistry
Analysis Of Mitochondrial Dna Coding Region Snps By Pyrosequencing, Kyle Robert Carl Parker
Analysis Of Mitochondrial Dna Coding Region Snps By Pyrosequencing, Kyle Robert Carl Parker
Electronic Theses and Dissertations
To date, the use of mitochondrial DNA in forensic analysis has relied on the presence of variations in the control region to differentiate between samples. One problem that this analysis has shown is the occurrence of common Haplogroup H haplotypes or identical sequences. Thus, there is a need to enhance the distinguishing power of this type of analysis. One option has been to investigate the mitochondrial coding region for polymorphisms that could differentiate between samples with identical control region haplotypes. The goal of this study has been to identify polymorphic coding region sites for development in a Pyrosequencing assay that …
Mitochondrial Dna Analysis By Pyrosequencing, Patsy-Ann Susan Hastings
Mitochondrial Dna Analysis By Pyrosequencing, Patsy-Ann Susan Hastings
Electronic Theses and Dissertations
Mitochondrial DNA (deoxyribo nucleic acid) is typically used in forensic casework when small quantities of high molecular weight quality DNA is not expected to be present thus negating the chances of obtaining usable nuclear DNA. Typical samples that utilized mitochondrial DNA analysis are: hair, bones, teeth, ancient remains (samples or remains that are at least 100 years old) or very old samples (samples that are less than 100 but greater than 10 years old). The current method used to evaluate mitochondrial DNA is Sanger sequencing. Although robust, it is also time consuming and labor intensive, on the other hand pyrosequencing …
The Use Of Pyrosequencing For The Analysis Of Y Chromosome Single Nucleotide Polymorphisms, Jeremy Charles Fletcher
The Use Of Pyrosequencing For The Analysis Of Y Chromosome Single Nucleotide Polymorphisms, Jeremy Charles Fletcher
Electronic Theses and Dissertations
The potential value of the Y chromosome for forensic applications has been recognized for some time with the current work dedicated to Short Tandem Repeat analysis and Single Nucleotide Polymorphism (SNP) discovery. This study examined the ability of two different SNP analysis methods to determine if they could be utilized in forensic applications and ultimately be developed into an established system for Y chromosome SNP analysis. This study examined two principle SNP analysis systems: single base extension and Pyrosequencing. Pyrosequencing was determined to be superior to single base extension, due to the wealth of information provided with sequencing and the …