Open Access. Powered by Scholars. Published by Universities.®
- Discipline
Articles 1 - 2 of 2
Full-Text Articles in Chemistry
Role Of Non-Muscle Myosin Ii In Hs578t/Hs578ts(I)8 Triple Negative Breast Cancer Progression, Shimara Gunawardana
Role Of Non-Muscle Myosin Ii In Hs578t/Hs578ts(I)8 Triple Negative Breast Cancer Progression, Shimara Gunawardana
Electronic Theses and Dissertations
Loss of cell-cell adhesion and migration are prerequisites for the formation of metastases. These events are mediated by changes in the actomyosin cytoskeleton. Nonmuscle myosin II (NMII) belongs to the myosin superfamily of motor proteins which binds to actin and controls actin cross-linking and contraction, however its role and regulation in cell-adhesion and migration remains poorly understood. In this study, we report that associations of α5β1integrin receptors and Ncadherins with NMII isoforms NM-IIA and NMII-B correlate with increased cell-cell adhesion and reduced migratory behavior of the parental Hs578T cells of the Hs578T/Hs578Ts(i)8 triple-negative breast cancer progression model. Using blebbistatin, a …
Functional Effects Of Calcium Regulation Of Thin Filaments At Single Particle Resolution, Christopher Solis-Ocampo
Functional Effects Of Calcium Regulation Of Thin Filaments At Single Particle Resolution, Christopher Solis-Ocampo
Electronic Theses and Dissertations
Heart disease is the leading cause of death in the United States. Understanding heart function at the molecular level is critical for developing of more effective treatments. In the cardiac muscle, the thin filament is composed by troponin (Tn), tropomyosin (Tm), and F-actin. It provides Ca2+-dependent regulation of contraction by modulating myosin attachment and force generation in a cooperative scheme. However, this mechanism remains unclear. To understand thin filament activation, we studied the binding and functional properties of Tn and Tm to F-actin at single particle resolution by employing fluorescence image colocalization, in vitro motility assays, and Förster resonance energy …