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Full-Text Articles in Chemistry
Fluorogenic Probes For Thioredoxin Reductase Activity, Tendai J. Mafireyi, Jorge O. Escobedo, Robert Strongin
Fluorogenic Probes For Thioredoxin Reductase Activity, Tendai J. Mafireyi, Jorge O. Escobedo, Robert Strongin
Chemistry Faculty Publications and Presentations
The thioredoxin system is vital in maintaining the cellular redox state and is implicated in various cancers and other diseases. It comprises of thioredoxin reductase, thioredoxin and NADPH. Various methods have been developed for the detection and quantification of the thioredoxin reductase enzyme. Most of these methods suffer the shortfall of poor specificity, hence there is need to develop more robust and specific techniques. Recently, there has been an increased interest in fluorescent probes that can target thioredoxin reductase. This review highlights the progress in the synthesis and application of the different fluorescent probes that have been employed for this …
Varied Length Stokes Shift Bodipy-Based Fluorophores For Multicolor Microscopy, Amy M. Bittel, Ashley M. Davis, Lei Wang, Michel A. Nederlof, Jorge O. Escobedo, Robert Strongin, Summer L. Gibbs
Varied Length Stokes Shift Bodipy-Based Fluorophores For Multicolor Microscopy, Amy M. Bittel, Ashley M. Davis, Lei Wang, Michel A. Nederlof, Jorge O. Escobedo, Robert Strongin, Summer L. Gibbs
Chemistry Faculty Publications and Presentations
Multicolor microscopy tools necessary to localize and visualize the complexity of subcellular systems are limited by current fluorophore technology. While commercial fluorophores cover spectral space from the ultraviolet to the near infrared region and are optimized for conventional bandpass based fluorescence microscopy, they are not ideal for highly multiplexed fluorescence microscopy as they tend to have short Stokes shifts, restricting the number of fluorophores that can be detected in a single sample to four to five. Herein, we synthesized a library of 95 novel boron-dipyrromethene (BODIPY)- based fluorophores and screened their photophysical, optical and spectral properties for their utility in …
A Seminaphthofluorescein-Based Fluorescent Chemodosimeter For The Highly Selective Detection Of Cysteine, Xiaofeng Yang, Yixing Guo, Robert M. Strongin
A Seminaphthofluorescein-Based Fluorescent Chemodosimeter For The Highly Selective Detection Of Cysteine, Xiaofeng Yang, Yixing Guo, Robert M. Strongin
Chemistry Faculty Publications and Presentations
A fluorescent chemodosimeter for cysteine detection was developed based on a tandem conjugate addition and intramolecular cyclization reaction. The method exhibited an excellent selectivity for cysteine over other biothiols such as homocysteine and glutathione.
Nir Dyes For Bioimaging Applications, Jorge O. Escobedo, Oleksandr Rusin, Soojin Lim, Robert M. Strongin
Nir Dyes For Bioimaging Applications, Jorge O. Escobedo, Oleksandr Rusin, Soojin Lim, Robert M. Strongin
Chemistry Faculty Publications and Presentations
Fluorescent dyes based on small organic molecules that function in the near infra red (NIR) region are of great current interest in chemical biology. They allow for imaging with minimal autofluorescence from biological samples, reduced light scattering and high tissue penetration. Herein, examples of ongoing NIR fluorophore design strategies as well as their properties and anticipated applications relevant to the bioimaging are presented.
Selective Fluorescence Detection Of Cysteine And N-Terminal Cysteine Peptide Residues, Soojin Lim, Jorge O. Escobedo, Mark Lowry, Xiangyang Xu, Robert M. Strongin
Selective Fluorescence Detection Of Cysteine And N-Terminal Cysteine Peptide Residues, Soojin Lim, Jorge O. Escobedo, Mark Lowry, Xiangyang Xu, Robert M. Strongin
Chemistry Faculty Publications and Presentations
A new fluorogenic fluorescein derivative containing an α,β-unsaturated aldehyde moiety produces a selective fluorescent signal enhancement in the presence of cysteine or peptides containing N-terminal cysteine residues. The mechanism is based on synergistic covalent and supramolecular interactions.
Optimising The Synthesis And Red–Green–Blue Emission Of A Simple Organic Dye, Martha Sibrian-Vazquez, Robert M. Strongin
Optimising The Synthesis And Red–Green–Blue Emission Of A Simple Organic Dye, Martha Sibrian-Vazquez, Robert M. Strongin
Chemistry Faculty Publications and Presentations
Synthetic dyes have been widely used in supramolecular chemistry not only to probe fundamental chemical interactions but also as components of functional materials. Most current efforts in this regard are directed at designing new host systems for the dyes. Herein we report on the study of a versatile new organic fluorophore. We describe a synthesis which affords improved yields in a convenient one pot procedure. Moreover, a simple method for predicting and controlling the dye’s responses to external stimuli affords a potentially practical method for achieving red–green–blue and concomitant white light generation.