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Full-Text Articles in Chemistry
Thermochemistry Of Fluorinated Aldehydes And Corresponding Radicals;Thermochemistry And Kinetics Of Diethyl Ether And Ethyl Oxirane Relative To Reactions Under Atmospheric And Combustion Conditions, Douglas Lee Purnell Jr.
Thermochemistry Of Fluorinated Aldehydes And Corresponding Radicals;Thermochemistry And Kinetics Of Diethyl Ether And Ethyl Oxirane Relative To Reactions Under Atmospheric And Combustion Conditions, Douglas Lee Purnell Jr.
Dissertations
Fundamental thermochemical properties including enthalpies (ΔH°f 298), entropies (S°(T)), heat capacities (Cp(T)), and bond dissociation energies (BDEs) for several common and complex hydrocarbon fuel species are determined using computational chemical methods. ΔH°f 298 values are calculated using isodesmic reactions with the CBS-APNO, CBS-4M, CBS-QB3, G2, G3, G4, Weizmann-1 (W1U) and M06-2X, ωB97X, B3-LYP with basis set 6-31G+ (d,p) and 6-31G++(d,p) calculation methods. Structures, moments of inertia, vibrational frequencies, and internal rotor potentials are calculated for contributions to entropies and heat capacities. Kinetic rate parameters are calculated for hydrogen abstraction and …
Protein Engineering Of Cota Laccase By Using Bacillus Subtilis Spore Display, Silu Sheng
Protein Engineering Of Cota Laccase By Using Bacillus Subtilis Spore Display, Silu Sheng
Dissertations
Spore display offers advantages over more commonly utilized microbe cell-surface display systems. For instance, protein-folding problems associated with the expressed recombinant polypeptide crossing membranes are avoided. Hence, a different region of protein space can be explored that previously was not accessible. In addition, spores tolerate many physical/chemical extremes. The aim is to improve pH stability using spore display. The maximum activity of CotA is between pH 4 and 5 for the substrate ABTS (ABTS = diammonium 2, 2’-azino-bis(3-ethylbenzothiazoline-6-sulfonate)). However, the activity dramatically decreases at pH 4. The activity is not significantly altered at pH 5. CotA is used as a …
Synthesis And Detection Of Dna Damage, Xun Gao
Synthesis And Detection Of Dna Damage, Xun Gao
Dissertations
The biological effects of DNA alkyl adducts are difficult to evaluate at the cellular level due to their instability. Synthesis of oligonucleotides that contain a single N7-alkylguanine has become a vital tool to achieve the above goal. However, the instability of N7-alkyguanines is not compatible with the phosphoramidite chemistry used by solid-phase oligonucleotide synthesis either. Development of chemically stable analogues of unstable DNA lesions enables accurate study of polymerase bypass. The design and successful synthesis of N7-hydroxyethyl-9-deaza-2′-deoxyguanosine and N7-oxoethyl-9-deaza-2′-deoxyguanosine as the stable analogues of N7-hydroxyethyl- 2'-deoxyguanosine and N7-oxoethyl-2′-deoxyguanosine, respectively, are reported. The synthesis of these two nucleosides whose N7 side …