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Full-Text Articles in Chemistry
Chemical Synthesis Of Sensitive Dna, Komal Chillar
Chemical Synthesis Of Sensitive Dna, Komal Chillar
Dissertations, Master's Theses and Master's Reports
Over the past decades, researchers have tried various chemical methods to synthesize modified oligodeoxynucleotides (ODNs, i.e. short segments of DNAs). Traditional ODN synthesis methods require strong basic, and nucleophilic conditions for the deprotection and cleavage of the ODN from the solid support. However, the sensitive ODNs containing labile functionalities are vulnerable to such harsh conditions. Sensitive ODNs have a wide range of applications in research and pharmaceuticals. To synthesize sensitive ODNs, researchers devised different strategies but no practical methods have been developed. To overcome these challenges, we developed alkyl Dim alkyl Dmoc technology. This innovative technology uses weakly basic and …
Determination Of Optical Density (Od) Of Oligodeoxynucleotide From Hplc Peak Area, Komal Chillar, Yipeng Yin, Dhananjani Eriyagama, Shiyue Fang
Determination Of Optical Density (Od) Of Oligodeoxynucleotide From Hplc Peak Area, Komal Chillar, Yipeng Yin, Dhananjani Eriyagama, Shiyue Fang
Michigan Tech Publications
Oligodeoxynucleotides (ODNs) are typically purified and analysed with HPLC equipped with a UV-Vis detector. Quantities of ODNs are usually determined using a UV-Vis spectrometer separately after HPLC, and are reported as optical density at 260 nm (OD260). Here, we describe a method for direct determination of OD260 of ODNs using the area of the peaks in HPLC profiles.
The Ion Pair Thermal Model Of Maldi Ms, Shiyue Fang
The Ion Pair Thermal Model Of Maldi Ms, Shiyue Fang
Michigan Tech Publications
The ion pair thermal model for MALDI MS is described. Key elements of the model include thermal desorption and ionization, strong tendency to neutralization via ion pair formation and proton transfer in the gas phase, thermal equilibrium, overall charge neutral plume, and thermal energy assisted free ion generation via ion pair separation by ion extraction potential. The quantities of ions in the solid sample and in the gaseous plume are estimated. Ion yields of different classes of molecules including peptides, nucleic acids, permanent salts and neutral molecules are estimated at the macroscale and single ion pair levels. The estimated ion …
Synthetic Oligodeoxynucleotide Purification Via Catching By Polymerization, Suntara Fueangfung
Synthetic Oligodeoxynucleotide Purification Via Catching By Polymerization, Suntara Fueangfung
Dissertations, Master's Theses and Master's Reports - Open
Large quantities of pure synthetic oligodeoxynucleotides (ODNs) are important for preclinical research, drug development, and biological studies. These ODNs are synthesized on an automated synthesizer. It is inevitable that the crude ODN product contains failure sequences which are not easily removed because they have the same properties as the full length ODNs. Current ODN purification methods such as polyacrylamide gel electrophoresis (PAGE), reversed-phase high performance liquid chromatography (RP HPLC), anion exchange HPLC, and affinity purification can remove those impurities. However, they are not suitable for large scale purification due to the expensive aspects associated with instrumentation, solvent demand, and high …