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Full-Text Articles in Chemistry
From Micro- To Nano-Scale: Applications Of Solid-Phase Enzymatic Reactors For Biopolymer Disassembly, Nyote J. Calixte
From Micro- To Nano-Scale: Applications Of Solid-Phase Enzymatic Reactors For Biopolymer Disassembly, Nyote J. Calixte
LSU Doctoral Dissertations
The process of immobilizing enzymes onto solid supports for bioreactions has some compelling advantages compared to their solution-based counterpart including the facile separation of enzyme from products, elimination of enzyme autodigestion, and increased enzyme stability and activity. We report in this work, the immobilization of λ-exonuclease onto poly(methylmethacrylate) (PMMA) micro- and nano-pillars populated within a fluidic devices for the micro and nanoscale on-chip digestion of double-stranded DNA. Enzyme immobilization in both studies was successfully accomplished using 3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling to carboxylic acid functionalized PMMA micropillars. Our micro-scale results suggest that the reaction efficiency for the catalysis of dsDNA digestion …