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Articles 1 - 30 of 102
Full-Text Articles in Chemistry
The Effect Of Autophagy Related Protein 10 And Its Mutants On Autophagic Activity, Sk Nadia Rahman Silvia
The Effect Of Autophagy Related Protein 10 And Its Mutants On Autophagic Activity, Sk Nadia Rahman Silvia
Senior Honors Theses and Projects
Autophagy is the cellular degradation process in which cellular contents are encapsulated by double-membrane vesicles, autophagosomes, and delivered to the vacuole to be degraded and recycled. This process is important for cell health and homeostasis. There are approximately 32 different autophagy-related proteins involved. Atg10 is an enzyme that may affect overall autophagic activity by changing autophagosome size and/or number. Our goal is to find mutants that cause a significant, partial loss in Atg10’s activity by mutating some residues near the active site and testing the functionality of these mutants by performing western blots and enzymatic assays. Here we show that …
Introducing An H113a Mutation Into Atg10 Y73q To Reduce Autophagic Activity And Understand The Effect Of Atg On Autophagosome Size And Number, Konrad L. Lautenschlager
Introducing An H113a Mutation Into Atg10 Y73q To Reduce Autophagic Activity And Understand The Effect Of Atg On Autophagosome Size And Number, Konrad L. Lautenschlager
Senior Honors Theses and Projects
Autophagy is an essential recycling process that occurs within eukaryotic cells, however, the individual functions of the current thirty-two known autophagic proteins are not yet entirely understood. At this time, it is known that the autophagic protein Atg7 works upstream of both Atg3 and Atg10. Atg3 is affected by Atg7 and allows for the attachment of Atg8 and the lipid PE which is part of the autophagosome membrane. The Atg10 pathway is also affected by Atg7 but attaches Atg12 to Atg5 which upregulates the function of Atg3 and ultimately Atg8 lipidation. These protein interactions are necessary for forming autophagosomes, large …
Analysis Of The Role Of Sgs1p In Repair Of Double-Strand Dna Breaks In Saccharomyces Cerevisiae, Beth Wasserman
Analysis Of The Role Of Sgs1p In Repair Of Double-Strand Dna Breaks In Saccharomyces Cerevisiae, Beth Wasserman
Senior Honors Theses and Projects
Mis-repair of DNA double-stranded breaks (DSB) can result in genomic rearrangements and cancer. Sgs1p is a helicase that unwinds DNA for DSB repair. To better understand its role in break repair, I analyzed the frequency of various types of DSB repairs in yeast cells with the mutation sgs1-FD. Given this mutation disrupts an interaction between Sgs1p and a critical protein, Rad51p, I hypothesized mutant cells would have more error-prone repair than high fidelity canonical BIR repair. Genome sequence and chromosome size were examined in sgs1-FD yeast cells that underwent DSB repair. No difference was found in the frequency of …
Polymer Chemistry Education To Increase Student Awareness Of Plastic Pervasiveness And Environmental Impact, Victoria Hill
Polymer Chemistry Education To Increase Student Awareness Of Plastic Pervasiveness And Environmental Impact, Victoria Hill
Master's Theses and Doctoral Dissertations
A polymer-pollution-focused laboratory sequence was written to teach general chemistry concepts in a course for pre-service elementary teachers. Students experienced the difficulties related to polymer pollution clean-up and made bio-based materials to see possible alternatives. In order to determine whether this laboratory sequence changed student understanding of the importance of polymers and their environmental impact, student opinions were gathered with pre/post-course sequence surveys, observations, post-laboratory surveys, and focus group interviews. These same data collection methods were also used to determine the likelihood that the pre-service elementary teachers would teach these polymer and environmental laboratories or their concepts in the future. …
Climate Change Instruction In Higher Education: Pre-Service Teachers’ Engagement In An Interdisciplinary Pop-Up Learning Community, Mary Charlotte Bautistia
Climate Change Instruction In Higher Education: Pre-Service Teachers’ Engagement In An Interdisciplinary Pop-Up Learning Community, Mary Charlotte Bautistia
Master's Theses and Doctoral Dissertations
An interdisciplinary pop-up learning community (PLC) allowed students from various disciplines with different levels of content knowledge to discuss their perspectives and beliefs on climate change. The impact of this event on students was gauged by a survey from the Yale Project on Climate Change Communication. Furthermore, this project focuses on pre-service teachers who participated in the PLC. In order to investigate how pre-service teachers understand climate change and how they may or may not integrate these issues into their own instruction, individual interviews were conducted prior to and after the PLC to determine if the event had an impact …
An Investigation Of The Nature Of Plasminogen Activator Inhibitor Type-I (Pai-I) To Improve Upon Known Small Molecule Inhibitors, Justin A. Powers
An Investigation Of The Nature Of Plasminogen Activator Inhibitor Type-I (Pai-I) To Improve Upon Known Small Molecule Inhibitors, Justin A. Powers
Senior Honors Theses and Projects
Plasminogen activator inhibitor type-I (PAI-I ) is a serpin superfamily member protein that acts as the major inhibitor of tissue-type plasminogen activator (tPA) and urokinase-type plasminogen activator (uPA). At physiological levels, PAI-I plays an active role in regulating fibrinolysis, the process of normal blood clot degradation. Pathological levels of PAI-I have been linked to multiple health ailments: fibrosis, heart disease, cancer, and obesity. In recent years, PAI-1 has been the subject of targeted drug design. In this study, we identified a class of polyphenol compounds as the most capable of maintaining its inhibitory efficiency against vitronectin bound PAI-I in buffer …
Uv-Curable Organic-Inorganic Hybrid Polyurethane Coating, Shiyin Yu
Uv-Curable Organic-Inorganic Hybrid Polyurethane Coating, Shiyin Yu
Master's Theses and Doctoral Dissertations
This thesis explored a series of organic-inorganic hybrid polyurethane oligomers with varying molecular weights and functional groups (acrylate and/or silane) that were cured with a single ultraviolet (UV) trigger but two photo-initiators. Upon UV irradiation, the free-radical initiator initiated a free-radical reaction for acrylate cross-linking, and the photo-latent base initiated a sol-gel reaction for condensation of the silanes. This study explored the cure extent of both reactions by FTIR for acrylates and silanes and a weight-loss method for silanes as a function of functional group concentration and time. The general film properties were also studied. The results showed that the …
Understanding The Role Of Atg7 And Atg14 In Autophagy, Ronith Chakraborty
Understanding The Role Of Atg7 And Atg14 In Autophagy, Ronith Chakraborty
Senior Honors Theses and Projects
Autophagy is the process by which cytosolic components are trafficked to and degraded by the vacuole or lysosome. It plays a critical role in cellular health, aging, cancer, and neurodegenerative diseases. Atg7 and Atgl4 are enzymes required for the autophagic process in Saccharomyces cerevisiae. In this study, we hypothesized that Atg7 controls the size while Atg 14 controls the number of autophagosomes. Using western blotting, Pho8D.60 assay and transmission electron microscopy analysis, we found that Atg7 affects both the size and number of autophagosomes. In addition, we have created cells expressing various levels of Atgl4 using non-native promoters. In addition, …
Polymerization Of Bromoundecylacrylate And Alkylating Methylimidazole, Shuziao Li
Polymerization Of Bromoundecylacrylate And Alkylating Methylimidazole, Shuziao Li
Master's Theses and Doctoral Dissertations
Polymerized ionic liquids (PIL) generally are solids, but a few examples have been reported that also exhibit liquidity in the neighborhood of room temperature called liquid polymerized ionic liquids (LPIL). The development of a platform suitable for exploring LPIL properties based on the monomer 11-bromoundecylacrylate (AcC11Br) was reported. Poly(AcC11Br) is itself a liquid polymer and can be easily used to alkylate 1-methylimidazole to produce poly(AcC11C1ImBr-co- AcC11Br). Various reaction conditions were explored to examine how extensively 1- methylimidazole can be attached to this polymer, and the extent of alkylation is determined quantitatively by silver ion potentiometry. These polymers retain liquidity at …
Synthesis And Study Of The Micellization Behavior Of A Dual Temperature- And Ph-Responsive Paa-B-Pnipam Block Copolymer, Ehsan Zarshenas Moghadam
Synthesis And Study Of The Micellization Behavior Of A Dual Temperature- And Ph-Responsive Paa-B-Pnipam Block Copolymer, Ehsan Zarshenas Moghadam
Master's Theses and Doctoral Dissertations
Amphiphilic diblock copolymers are composed of hydrophilic and hydrophobic regions. When they are exposed to water, the hydrophobic blocks aggregate to form a hydrophobic core surrounded by hydrophilic regions outside to give a core-shell micelle. These micelles have many potential applications, such as a drug delivery system, surfactants, or nanoreactor. Many studies have been done to optimize the performance of these copolymers. In this thesis work, nine dual temperature- and pH-responsive poly(acrylic acid-block-N-isopropylacrylamide) (PAA-b-PNIPAM) diblock copolymers with different relative chain lengths of the hydrophobic and hydrophilic blocks were synthesized by reversible addition fragmentation chain transfer polymerization. The preliminary results showed …
Polymerized Ionic Liquids By Condensation Polymerization: Stimuli Responsive Polyurethane Gels And Dispersions, Harshit Gupta
Polymerized Ionic Liquids By Condensation Polymerization: Stimuli Responsive Polyurethane Gels And Dispersions, Harshit Gupta
Master's Theses and Doctoral Dissertations
Ionic liquids have been used in free radical polymerizations to make polymerized ionic liquid (PIL) materials of various types. PIL gels based on imidazolium cations have been found to exhibit an anion-exchange induced stimuli responsiveness. This thesis explores incorporation of ionic liquids in polyurethane (PU) polymers to make PIL PU gels and dispersions through condensation polymerization. PIL gels are synthesized through a single-pot approach that show stimuli response to solvents. This approach allows one to make these gels rapidly and cheaply on-site. These gels can reversibly porate in different solvents and are found to be porous when analyzed by scanning …
Design,Formulation And Synthesis Of Uv/Thermal Dual Cure, Bio-Based Polyurethane Coatings, Ozlem Kubra Akdogan
Design,Formulation And Synthesis Of Uv/Thermal Dual Cure, Bio-Based Polyurethane Coatings, Ozlem Kubra Akdogan
Master's Theses and Doctoral Dissertations
In the present work, polyurethane resins have been synthesized using soybean oil based polyols (> 80% bio-renewable content) as a primary component. To overcome some of the limitations associated with soft and flexible structure of soy-based polyols, the resin has been designed with two unique set of functional groups –OH and acrylate, as a means of crosslinking sites. Such resin system can be cured by two independent cure mechanisms – (a) thermal (or ambient) curing of –OH groups by use of isocyanate cross-linkers, and (b) by UV-curing of acrylate functional groups using appropriate photoinitiators. Thus, this dual-curable system can provide …
Characterization Of The Histone Binding Properties Of The Epigenetic Reader Protein Uhrf2 To H3 In Phd Domain, Marisa Gilliam
Characterization Of The Histone Binding Properties Of The Epigenetic Reader Protein Uhrf2 To H3 In Phd Domain, Marisa Gilliam
Senior Honors Theses and Projects
Every cell contains the same genetic code, to have cell differentiation this genetic code needs to be regulated on what is expressed. The regulation of gene expression without altering the genetic code itself is known as epigenetics. An example of epigenetic regulation can be seen between the binding of UHRF2 and H3 histones. The purpose of this project is to determine ifD363 in UHRF2 is important for histone H3 binding. To do so, D363 was mutated to alanine, lysine or asparagine. The mutant protein was expressed, purified, and its ability to binding to H3 was tested by fluorescence polarization. Compared …
Synthesis And Catalytic Testing Of A Proline Functionalized Amphiphilic Block Copolymer, Eric Firestone
Synthesis And Catalytic Testing Of A Proline Functionalized Amphiphilic Block Copolymer, Eric Firestone
Master's Theses and Doctoral Dissertations
Catalysts are reagents used to reduce the amount of energy that a reaction needs to execute, allowing it to proceed at a higher rate while not being consumed. The use of catalyst functionalized amphiphilic copolymer allows easier recovery of the catalyst at the end of the synthesis and allows for the reaction. In water, under appropriate conditions, the amphiphilic block copolymers will form micelles and allow the reaction to occur on the interior of the micelle. The synthesis of amphiphilic copolymer with a hydrophobic O-methacryloyl-trans-4- hydroxy-L-proline and methyl methacrylate end and a hydrophilic acrylic acid end was characterized. The polymer …
Determining Atg11'S Binding Interactions Using A Yeast Two Hybrid System And Directed Mutagenesis, Jesse Smith
Determining Atg11'S Binding Interactions Using A Yeast Two Hybrid System And Directed Mutagenesis, Jesse Smith
Senior Honors Theses and Projects
Autophagy is a mechanism of intracellular degradation within eukaryotes. Because of its aid to a cells longevity, autophagy is believed to be important for the prevention of neurodegenerative diseases. Autophagy related protein 11 (Atg11) is a coiled-coil scaffolding protein required for selective autophagy in yeast. Atg11 is known to interact with Atg1, Atg20, and Atg29, in addition to connecting cargo molecules prApel -Atg19 to the phagophore assembly site. In addition to its many functions within yeast autophagy, Atg11 has two human homologs: Huntingtin protein and FIP200. This homology encourages us to gain a further understanding of Atg11's binding sites. In …
Effects Of Yoga On The Motor Skills Of A Ten-Year-Old Male With Autism Spectrum Disorder, Amber Schmitt
Effects Of Yoga On The Motor Skills Of A Ten-Year-Old Male With Autism Spectrum Disorder, Amber Schmitt
Senior Honors Theses and Projects
Autism Spectrum Disorder (ASD} is a neurodevelopmental disorder that is typically diagnosed in childhood due to the presence of atypical development, social interactions, and repetitive stereotypical patterns of behavior. The purpose of the present study was to examine the effects of a yoga intervention on a ten-year-old male diagnosed with ASD. It was hypothesized that the yoga intervention on select instructed poses would increase both pose duration and pose performance and that the results would generalize to longer pose duration and better pose performance on non-instructed poses. Pre-intervention assessments of motor functioning were administered, including the Movement ABC, BOT-2, TGMD, …
Analysis Of Oxidation Of Carbon Monoxide On Rh (110), Razan Tanbour
Analysis Of Oxidation Of Carbon Monoxide On Rh (110), Razan Tanbour
Senior Honors Theses and Projects
81% of all rhodium (Rh) is used in automobile catalytic converters. Consequently, researchers have dedicated a lot of effort into understanding the behavior of nonmetal oxides on a Rh surface. Using a computer simulation program, we have studied the absorption and oxidation of carbon monoxide (CO) on a terraced Rh surface (lattice) with a width of three and a height of 10000 using a mechanism that describes the behavior at low temperatures. Varying the reaction activation energies (rate constants), and the mobility of the CO, led to changes in the surface coverages of oxygen and CO. Specifically, increasing the activation …
Understanding The Interface That Enables The Interaction Between Two Dopamine Receptor Subtypes Known As D1 And D2 Implicated In Depression, Adam Baraka
Senior Honors Theses and Projects
The dopamine receptor subtypes known as DI and D2 have been shown to form a heteromer complex, which is thought to lead to the disruption of a multitude of signaling pathways within the brain. As a result, this has been proposed to lead to diseased states such as Alzheimer's and depression. In previous studies, it has been shown that the use of synthetic peptides corresponding to the surface interface of the interactions (specifically in the third intracellular loop of D2 interface) within the complex have served to compete against the formation of the heteromer. Through in vitro biochemical techniques such …
Analysis Of Variable Regions In Feline Leukemia Virus Envelope Sequences, Brianna Sohl
Analysis Of Variable Regions In Feline Leukemia Virus Envelope Sequences, Brianna Sohl
Senior Honors Theses and Projects
Feline Leukemia Virus (FeLV) is a retrovirus that affects domesticated cats and other species in the Felis genus. There are three major subtypes, each characterized by unique disease progression and proviral sequences. FeLV-A is weakly pathogenic, -B has been associated with tumors, and -C causes severe anemia. Proviral sequences were amplified using PCR with FeL V-specific and adapter-specific primers. The method of primer walking has yielded consensus sequences for several fragments of 1,000-3,000 base pairs of proviral DNA. Sequences for ten different clones of the provirus envelope region from a FeL V positive blood sample were compared to known sequences …
The Anticancer Effects Of The Different Analogs Of Cysteine Deleted Tachyplesin On A549 Lung Cancer Cells, Nathan Riley Hendrickson
The Anticancer Effects Of The Different Analogs Of Cysteine Deleted Tachyplesin On A549 Lung Cancer Cells, Nathan Riley Hendrickson
Master's Theses and Doctoral Dissertations
It has been previously shown that cysteine-deleted tachyplesin (CDT) and its many analogs display antibacterial effects. However, little has been said about its possible anticancer effects and which analogs serve as the best inhibitors of the growing cancer cells. This research focused on CDT and four of its analogs: All D-CDT, Reverse DCDT, Reverse CDT, and Serine-Control CDT. MTT assays against the A549 adenocarcinoma lung cancer cell line showed All D-CDT to be the most effective analog in preventing cancer cell growth. Hyaluronidase and apoptosis assays were performed to better understand the mechanism by which the All D-CDT is preventing …
Expression And Purification Of Atg11: A Protein Involved In The Organization Of Autophagy, Carson Zois
Expression And Purification Of Atg11: A Protein Involved In The Organization Of Autophagy, Carson Zois
Senior Honors Theses and Projects
Autophagy is a physiological process for the degradation of internal cell components to promote cell survival. This process allows the cell to maintain homeostasis, recycle damaged cellular components and helps protect against disease. Autophagy can be selective or non-selective. Non-selective, or bulk, autophagy occurs during times of cellular starvation and results in non-specific degradation of cytoplasmic materials. Selective autophagy targets specific components within the cytoplasm for degradation, and is always active. In both forms of autophagy, components are degraded when a double-membraned structure, called the autophagosome, encapsulates the target cargo and is then recruited to the lysosome in mammalian cells, …
Investigation Of Atg11 And Its Interaction With Ypt1 In Autophagy, Hayley Cawthon
Investigation Of Atg11 And Its Interaction With Ypt1 In Autophagy, Hayley Cawthon
Senior Honors Theses and Projects
Autophagy is a cellular process conserved in eukaryotes that involves the trafficking of intracellular material from the cytosol to the vacuole/lysosome. This material is wrapped in a double membraned vesicle called an autophagosome, which ls constructed at the pre-autophagosomal structure. Many proteins are Involved In autophagy, one of them being Atgll. This protein is crucial in the selective autophagy pathway, and is responsible for proper formation of the pre-autophagosomal structure as well as recruiting the material intended for degradation to the autophagosome. Atgl 1 interacts with many other autophagy proteins, but it Is unknown whether it interacts with these binding …
Synthesis And Structure Activity Relationship Of Novel Small Molecules As Inhibitors Of Plasminogen Activator Inhibitor-1, Karl Michael Upman
Synthesis And Structure Activity Relationship Of Novel Small Molecules As Inhibitors Of Plasminogen Activator Inhibitor-1, Karl Michael Upman
Master's Theses and Doctoral Dissertations
Plasminogen activator inhibitor type-1 (PAI-1) is a member of the serine protease inhibitor (serpin) family. PAI-1 is involved in the regulation of fibrinolysis, which is the breakdown of blood clots. PAI-1 inhibits serine proteases tissue-type plasminogen activator (tPA) and urokinase-type plasminogen activator (uPA), which are key factors in fibrinolysis. Excess levels of PAI-1 have also been shown to increase the risk of diabetes, stroke, and atherosclerosis, as well as tumor development. Previous attempts at synthesizing a practical drug for the regulation of PAI-1 have not been successful; therefore, it has been the purpose of this study to further advance progress …
Diastereoselective Aza-Cope Rearrangement-Mannich Cyclizations Of Conformationally Mobile Iminium Cations, Joshua Hunt
Diastereoselective Aza-Cope Rearrangement-Mannich Cyclizations Of Conformationally Mobile Iminium Cations, Joshua Hunt
Master's Theses and Doctoral Dissertations
We have developed a Lewis-acid catalyzed, diastereoselective aza-Cope rearrangement— Mannich cyclization (ACM) to form acyl pyrrolidines from conformationally mobile substrates. Earlier studies from our lab have shown that substituted acyl pyrrolidines can be synthesized with diastereoselectivity of 50:1 trans to cis, and that unsubstituted pyrrolidines can be synthesized with 13:1 enantioselectivity. This study attempted to observe the stereochemical outcomes of the ACM for disubstituted pyrrolidine systems using oxazolidine starting materials. A proof of concept dimethyl-substituted oxazolidine was subjected to ACM conditions, producing the pyrrolidine with good yields. However, when methyl-phenyl substituted oxazolidine was subjected to ACM conditions, undesired byproduct …
Synthesis Of Dmap Tethered Polymer Chains For Reversible Micelle Formation, Richard Arthur Fredricks
Synthesis Of Dmap Tethered Polymer Chains For Reversible Micelle Formation, Richard Arthur Fredricks
Master's Theses and Doctoral Dissertations
Dimethylaminopyridine (DMAP) can act to catalyze numerous organic reactions. To increase its recovery and reuse, it has been tethered to dendrimers and to micellar systems, creating nano-scale catalytic pockets for reactions to proceed. Previously developed systems show diminished reactivity over time as the products of the reaction fill up the nano-scale reactor. Thus, the goal of my research is to use RAFT polymerization methods to synthesize a hydrophobic polymer chain bearing 4-methylaminopyridine units and to attach it to a hydrophilic polymer chain to create a polymer system capable of micelle formation in solution. These functionalized micelles are designed to be …
Progress Toward A Structure-Function Relationship For Novel, Small-Molecule Inhibitors Of Plasminogen Activator Inhibitor-1, Sarah Burke
Master's Theses and Doctoral Dissertations
Plasminogen activator inhibitor-1 (PAI-1) is a serpin protein whose function is to inhibit tissue-type plasminogen activator (tPA) and urokinase-type plasminogen activator (uPA), and hence, fibrinolysis. This inhibition leads to decreased plasminogen to plasmin conversion, which results in deleterious effects on the organism, such as blood clots. Elevated levels of PAI-1 are implicated in a variety of diseases and conditions. We have synthesized a variety of novel, small-molecule PAI-1 inhibitors in order to establish a structure-function relationship throughout the compounds. This goal was accomplished through an iterative process in which only one aspect of the molecule was altered at a time. …
Modeling Volatile Organic Compound Emission From Materials Used In Passenger Vehicle Interiors, Shane Canaday
Modeling Volatile Organic Compound Emission From Materials Used In Passenger Vehicle Interiors, Shane Canaday
Master's Theses and Doctoral Dissertations
Travel is an everyday necessity for many people, making the environment of a passenger vehicle a place where they spend a significant amount of time. Previous studies have indicated that more than 100 volatile organic compounds (VOCs) are present in ambient air within the cabins of new cars, some of which have been found to have adverse health effects. While previous VOC models have been produced, there is still uncertainty in these models with respect to changing variables such as temperature, sunlight, and the presence of multiple VOCs. An accurate and reliable model, capable of determining the concentration of different …
Using Peptides To Examine The Interaction Interface Between Aspartate Transcarbamoylase And Dihydroorotase In Pyrimidine Biosynthesis In Aquifex Aeolicus, Nouf Alyami
Master's Theses and Doctoral Dissertations
Aspartate transcarbamoylase (ATCase) and Dihydroorotase (DHOase) catalyze the second and third steps, respectively, in de novo pyrimidine biosynthesis. Both enzymes form an active complex (DAC) in Aquifex aeolicus, where loop A of DHOase interacts with a domain of ATCase. The main objective of this work is to determine the function of specific residues of loop A in DAC interactions and to alter the catalytic activities through disruption of the interface between the two enzymes from A. aeolicus. The ATCase and DHOase domains have been expressed in Escherichia coli and purified using affinity chromatography. The interface of the published …
Microwave-Assisted Synthesis Of Ss-Amino Alcohols, Hinalbahen Sanket Desai
Microwave-Assisted Synthesis Of Ss-Amino Alcohols, Hinalbahen Sanket Desai
Master's Theses and Doctoral Dissertations
ß-amino alcohols are common substructures in many biologically active compounds and can also be used as catalysts for asymmetric reactions. One common method for forming these moieties is by ring opening of unsymmetrical epoxides using amines via a substitution reaction. However, the majority of these methods requires excess of reagents, inorganic initiators, and/or extended reaction times. We have developed an efficient, regioselective route to synthesize amino alcohols via microwave-assisted aminolysis of several hindered and unhindered epoxides using amine nucleophiles of varying strengths. Microwave reactions can be done without Lewis acids or promoters, even for the most hindered trisubstituted epoxides. In …
Determination Of Thiamine In Solution By Uv-Visible Spectrophotometry: The Effect Of Interactions With Gold Nanoparticles, Michael Vincent Zielinski
Determination Of Thiamine In Solution By Uv-Visible Spectrophotometry: The Effect Of Interactions With Gold Nanoparticles, Michael Vincent Zielinski
Master's Theses and Doctoral Dissertations
The method presented here provides the foundation for a simple and selective qualitative determination of thiamine in solution. Gold nanoparticles in the presence of thiamine results in the formation of a secondary peak in the absorbance spectrum of the mixture. This peak can be used as an indicator of thiamine, which is useful for the qualitative analysis of solutions, and may provide an alternative to other methods for evaluating thiamine in blood and other biological systems. This method uses gold nanoparticles of a size around 20 to 30 nm and involves their selective interaction with thiamine, compared to selected amino …