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Full-Text Articles in Chemistry
Inducible Nitric Oxide Synthase Embedded In Alginate/Polyethyleneimine Hydrogel As A New Platform To Explore No-Driven Modulation Of Biological Function, Shaimaa Maher, Lauren A. Smith, Celine A. El-Khoury, Haitham F. Kalil, Khalid Sossey-Alaoui, Mekki Bayachou
Inducible Nitric Oxide Synthase Embedded In Alginate/Polyethyleneimine Hydrogel As A New Platform To Explore No-Driven Modulation Of Biological Function, Shaimaa Maher, Lauren A. Smith, Celine A. El-Khoury, Haitham F. Kalil, Khalid Sossey-Alaoui, Mekki Bayachou
Chemistry Faculty Publications
Nitric oxide (NO), a small free radical molecule, turned out to be pervasive in biology and was shown to have a substantial influence on a range of biological activities, including cell growth and apoptosis. This molecule is involved in signaling and affects a number of physiologic functions. In recent decades, several processes related to cancer, such as angiogenesis, programmed cell death, infiltration, cell cycle progression, and metastasis, have been linked with nitric oxide. In addition, other parallel work showed that NO also has the potential to operate as an anti-cancer agent. As a result, it has gained attention in cancer-related …
Nitric Oxide Synthase Encapsulation In Liposomes: A Potential Delivery Platform To (Nitric Oxide)-Deficient Targets, Reshani H. Perera, Pubudu M. Peiris, Serban F. Peteu, Mekki Bayachou
Nitric Oxide Synthase Encapsulation In Liposomes: A Potential Delivery Platform To (Nitric Oxide)-Deficient Targets, Reshani H. Perera, Pubudu M. Peiris, Serban F. Peteu, Mekki Bayachou
Chemistry Faculty Publications
Nitric oxide (NO) is a freely diffusible, gaseous free radical, associated with many physiological and pathological processes: such as neuronal signaling, immune response and inflammatory response. In mammalian organisms, NO is produced from L-arginine in an NADPH-dependent reaction catalyzed by a family of nitric oxide synthase (NOS) enzymes. Typically, large NO fluctuations in biological systems under/over a critical limit is associated with problems that range from transient dysfunctions to severe chronic disease states. In this regard, we explore the development of a potential delivery and release method of nitric oxide to NO-deficient sites using liposomes as vehicles. Liposomes have already …
Cholesterol Levels And Activity Of Membrane Bound Proteins: Characterization By Thermal And Electrochemical Methods, Noufissa Zanati, Michael Ellen Mathews, Perera N. Indika, John J. Moran, Jean A. Boutros, Alan T. Riga, Mekki Bayachou
Cholesterol Levels And Activity Of Membrane Bound Proteins: Characterization By Thermal And Electrochemical Methods, Noufissa Zanati, Michael Ellen Mathews, Perera N. Indika, John J. Moran, Jean A. Boutros, Alan T. Riga, Mekki Bayachou
Chemistry Faculty Publications
The long-term goal of this investigation is to study the effects of increased cholesterol levels on the molecular activity of membrane-bound enzymes such as nitric oxide synthase, that are critical in the functioning of the cardiovascular system. In this particular investigation, we used differential scanning calorimetry (DSC) and dielectric thermal analysis (DETA) to study the effect of added cholesterol on melting/recrystallization and dielectric behavior, respectively, of phosphatidylcholine (PC) bilayered thin films. We also used electrochemical methods to investigate the effect of added cholesterol on the redox behavior of the oxygenase domain of nitric oxide synthase as a probe embedded in …
Hemeproteins Bathed In Ionic Liquids;Examining The Role Of Water And Protons In Redox Behavior And Catalytic Function, John Joseph Moran
Hemeproteins Bathed In Ionic Liquids;Examining The Role Of Water And Protons In Redox Behavior And Catalytic Function, John Joseph Moran
ETD Archive
We investigate the changing behaviors of myoglobin and nitric oxide synthase (NOS) in the near-absence of bulk water and/or protons by using ionic liquid butyl methyl imidazolium tetrafluoroborate as a non-aqueous milieu. Through direct charge transfer and metalloprotein-mediated catalytic reduction of oxygen and nitric oxide, we shed light on diverging aspects on how the two hemeproteins face the scarcity of water and/or protons in bulk. Isotopic effect investigations using D2O further elucidates kinetic aspects of proton transfer. Finally, in the case of NOS oxygenase, pterin cofactor binding and NOS-mediated catalytic oxidation of L-arginine in ionic liquids interrogates proton and water …
New Generation Of Electrochemical Sensors For Nitric Oxide;Ruthenium/Carbon-Based Nanostructures And Colloids As Electrocatalytic Platforms, W. Pubudu M. Peiris
New Generation Of Electrochemical Sensors For Nitric Oxide;Ruthenium/Carbon-Based Nanostructures And Colloids As Electrocatalytic Platforms, W. Pubudu M. Peiris
ETD Archive
Nitric oxide (NO) is an important intercellular messenger that acts in many tissues to regulate a diverse range of physiological and pathological processes. The physiologically implications of NO function are far from being completely understood. The multifaceted reactivity of NO prompted the need for accurate determination of the concentration of this molecule. However, it is difficult to detect nitric oxide, particularly in biological media and near live cells due to its short half-life, a result of its reactivity and the low levels of NO produced in vivo. As a result, the accurate and reliable detection of NO under varying experimental …
Nitric Oxide Synthase In Confined Environments;Detection And Quantification Of Nitric Oxide Released From Cells And Modified Liposomes Using A Sensitive Metal Catalyst-Pedot Modified Carbon Fiber Electrode, P. A. Reshani H. Perera
Nitric Oxide Synthase In Confined Environments;Detection And Quantification Of Nitric Oxide Released From Cells And Modified Liposomes Using A Sensitive Metal Catalyst-Pedot Modified Carbon Fiber Electrode, P. A. Reshani H. Perera
ETD Archive
Nitric oxide (NO) is a freely diffusible, gaseous free radical, associated with many physiological and pathological processes that include neuronal signaling, immune response, and inflammatory response. NO is produced from L-arginine in an NADPH-dependent reaction catalyzed by a family of nitric oxide synthase (NOS) enzymes. A deficiency in NO plays a role in hypertension, hyperglycaemia, and arteriosclerosis, among other pathological states. Conversely, increased NO levels contribute to arthritis, septic shock, and hypotention. Therefore, measuring and quantifying NO production in biological systems and matrices may be vital in elucidating physiological and pathological processes. The goal of this work is to develop …