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Amino Acid Region 1000–1008 Of Factor V Is A Dynamic Regulator For The Emergence Of Procoagulant Activity, Joesph R. Wiencek, Mahesheema Na, Jamila Hirbawi, Michael Kalafatis
Amino Acid Region 1000–1008 Of Factor V Is A Dynamic Regulator For The Emergence Of Procoagulant Activity, Joesph R. Wiencek, Mahesheema Na, Jamila Hirbawi, Michael Kalafatis
Chemistry Faculty Publications
Single chain factor V (fV) circulates as an Mr 330,000 quiescent pro-cofactor. Removal of the B domain and generation of factor Va (fVa) are vital for procoagulant activity. We investigated the role of the basic amino acid region 1000-1008 within the B domain of fV by constructing a recombinant mutant fV molecule with all activation cleavage sites (Arg(709)/Arg(1018)/Arg(1545)) mutated to glutamine (fV(Q3)), a mutant fV molecule with region 1000-1008 deleted (fV(ΔB9)), and a mutant fV molecule containing the same deletion with activation cleavage sites changed to glutamine (fV(ΔB9/Q3)). The recombinant molecules along with wild type fV (fV(WT)) were transiently expressed …