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Full-Text Articles in Chemistry
The Low-Resolution Structure Of Nascent High Density Lipoprotein Reconstituted With Dmpc With And Without Cholesterol Reveals A Mechanism For Particle Expansion, Valentin Gogonea, Gary S. Gerstenecker, Zhiping Wu, Xavier Lee, Celalettin Topbas, Matthew A. Wagner, Thomas C. Tallant, Jonathan D. Smith, Phil Callow, Vitaliy Pipich, Helen Malet, Guy Schoehn, Joseph A. Didonato, Stanley L. Hazen
The Low-Resolution Structure Of Nascent High Density Lipoprotein Reconstituted With Dmpc With And Without Cholesterol Reveals A Mechanism For Particle Expansion, Valentin Gogonea, Gary S. Gerstenecker, Zhiping Wu, Xavier Lee, Celalettin Topbas, Matthew A. Wagner, Thomas C. Tallant, Jonathan D. Smith, Phil Callow, Vitaliy Pipich, Helen Malet, Guy Schoehn, Joseph A. Didonato, Stanley L. Hazen
Chemistry Faculty Publications
High density lipoproteins (HDL) are athero-protective particles under investigation as potential therapeutic agents for cardiovascular disease. We applied small angle neutron scattering (SANS) with contrast variation to obtain the low resolution structure of nascent HDL (nHDL) reconstituted with dimyristoyl phosphatidyl choline (DMPC), apoA1:DMPC (1:80, mol:mol). The overall shape of the entire particle is discoidal, with low resolution architecture of apoA1 visualized as an open, contorted, and slightly out of plane structure with three arms, while the low resolution shape of the lipid phase is an oblate ellipsoid that fits well within the protein shape. Modeling studies incorporating the SANS data …
The Low Resolution Structure Of Apoa1 In Spherical High Density Lipoprotein Revealed By Small Angle Neutron Scattering, Zhiping Wu, Valentin Gogonea, Xavier Lee, Roland P. May, Vitaliy Pipich, Matthew A. Wagner, Arundhati Undurti, Thomas C. Tallant, Camelia Baleanu Gogonea, Francesca Charlton, Alexander I. Ioffe, Joseph A. Didonato, Kerry Anne Rye, Stanley L. Hazen
The Low Resolution Structure Of Apoa1 In Spherical High Density Lipoprotein Revealed By Small Angle Neutron Scattering, Zhiping Wu, Valentin Gogonea, Xavier Lee, Roland P. May, Vitaliy Pipich, Matthew A. Wagner, Arundhati Undurti, Thomas C. Tallant, Camelia Baleanu Gogonea, Francesca Charlton, Alexander I. Ioffe, Joseph A. Didonato, Kerry Anne Rye, Stanley L. Hazen
Chemistry Faculty Publications
Spherical high density lipoprotein (sHDL), a key player in reverse cholesterol transport and the most abundant form of HDL, is associated with cardiovascular diseases. Small angle neutron scattering with contrast variation was used to determine the solution structure of protein and lipid components of reconstituted sHDL. Apolipoprotein A1, the major protein of sHDL, forms a hollow structure that cradles a central compact lipid core. Three apoA1 chains are arranged within the low resolution structure of the protein component as one of three possible global architectures: (i) a helical dimer with a hairpin (HdHp), (ii) three hairpins (3Hp), or (iii) an …